2024-05-10
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113547319
External Id
Instituto de Investigacion Sanitaria del Principado de Asturias (ISPA)
INSDC center name
2023-05-24T16:14:07Z
INSDC last update
public
INSDC status
ERS15543740
SRA accession
E-MTAB-13008:Sample 11
Submitter Id
51
year
age
ArrayExpress
broker name
human
common name
Protocols: Fresh visceral adipose tissue and skeletal muscle were obtained simultaneously during the bariatric surgery procedure under sterile conditions and immediately transported to the laboratory. A laminar flux cabin was used for tissue manipulation to avoid contamination and blood vessels were cleaned out by using tweezers and scissors. Then, samples were frozen at -80oC immediately total RNA of each sample was extracted from 50-100 mg of tissue by using trizol reagent. Total RNA of each sample was used to prepare the miRNA sequencing library. After the completed libraries were quantified with Agilent 2100 Bioanalyzer, the DNA fragments in the libraries were denatured with 0.1M NaOH to generate single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ and finally sequenced for 51 cycles on Illumina NextSeq 500 according to the manufacturer’s instruction. The libraries were denatured as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 51 cycles on Illumina NextSeq per the manufacturer’s instructions.
description
adult
developmental stage
morbid obesity
disease
P034
individual
Homo sapiens
organism
Homo sapiens
organism
visceral abdominal adipose tissue
organism part
Homo sapiens
scientific_name
female
sex
Sample 11
title