2024-05-11
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113547084
External Id
SHANGHAI JIAO TONG UNIVERSITY
INSDC center name
2023-05-24T14:37:09Z
INSDC last update
public
INSDC status
ERS15543505
SRA accession
E-MTAB-13003:Sample 3
Submitter Id
ArrayExpress
broker name
grass carp
common name
Protocols: The CIK cells were maintained on RPMI1640 supplemented with 10% FBS, 1% penicillin-streptomycin. The genomic DNA was extracted from the cells and fragmented to ∼300 bp using a Covaris M220 sonicator. Fragmented DNA was end-repaired, A-tailed, and ligated to Illumina multiplexing adapters following the standard protocol of KAPA Hyper Prep kits.Adapter-ligated DNA was denatured at 95°C and then immunoprecipitated overnight at 4°C with 1 µl antibody. The immunoprecipitated DNA was amplified for approximately 12-15 cycles, and fragments of the proper size (usually 300-500 bp) were gel-purified using the Gel Extraction Kit (zymo research) and quantified using a 2100 Bioanalyzer (Agilent Technologies).
description
adult
developmental stage
normal
disease
wild type genotype
genotype
Ctenopharyngodon idella
organism
Ctenopharyngodon idella
organism
kidney
organism part
Ctenopharyngodon idella
scientific_name
Sample 3
title