2024-05-13
ENA first public
ERC000011
ENA-CHECKLIST
Dipartimento di Medicina Molecolare e Biotecnologie Mediche (DMMBM), Università Federico II di Napoli; CEINGE Biotecnologie Avanzate Franco Salvatore
INSDC center name
public
INSDC status
ERS18458674
SRA accession
E-MTAB-13916:HEK_CRISPR_2
Submitter Id
ArrayExpress
broker name
HEK293T
cell_line
not collected
collection date
human
common name
Protocols: Edited (C/C) and unedited (T/T) Hek293T cells (at 75% of confluency) were lysed with TRIzol RNA Isolation Reagent (#15596018; Ambion, Thermo Fisher Scientific) and frozen at -80°C. HEK293T cells were cultured using Dulbecco's modified Eagle's medium (DMEM; 41966-029; Gibco) with 10% fetal bovine serum 10270-106; Gibco), 2 mM L-glutamine (25030-024; Gibco), and 1% penicillin/streptomycin (P0781; Sigma-Aldrich), with the medium changed daily.  Total RNA, isolated by using TRIzol RNA Isolation Reagent (#15596018; Ambion, Thermo Fisher Scientific) was quantified in a NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermo Scientific™), and checked for purity and integrity. Libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA for Sequencing, Nextera XT DNA Library Preparation Kit according to the protocols recommended by the manufacturer. Library kit: TSMART-Seq v4 Ultra Low Input RNA for Sequencing, Nextera XT DNA Library Preparation Kit; Library protocol: SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing User Manual, Nextera XT DNA Library Prep Kit Reference.
description
crispr edited
genetic modification
crispr edited
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
kidney
organism part
Homo sapiens
scientific_name
HEK_CRISPR_2
title