2024-05-14
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114579215
External Id
IOW Leibniz-Institut fur Ostseeforschung Warnemuende
INSDC center name
2023-11-06T10:23:55Z
INSDC last update
public
INSDC status
ERS16667787
SRA accession
130978:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67
Submitter Id
GFBIO
broker name
2021-08-01
collection date
Seagrass leave biofilm from station BV11-A, replicate c
description
temperate marginal sea biome [ENVO:01000856]
environment (biome)
sea grass bed [ENVO:01000059]
environment (feature)
plant matter [ENVO:01001121]
environment (material)
host-associated
environmental package
Yes
environmental_sample
lib_3
experiment
Germany
geographic location (country and/or sea)
2.2
m
geographic location (depth)
54.1393
DD
geographic location (latitude)
13.60813
DD
geographic location (longitude)
Coastal samples
geographic location (region and locality)
host-associated
host-associated environmental package
mimarks-specimen
investigation type
Samples were thawed and homogenized. A subsample of 4-6 leaves was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit
nucleic acid extraction
biofilm metagenome
organism
FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC
pcr primers
16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments
project name
B
run
Stored at -80 C
sample material processing
Seagrass leave biofilm from station BV11-A, replicate c
sample_description
biofilm metagenome
scientific_name
Illumina MiSeq
sequencing method
true
submitted to insdc
V3-V4
target subfragment
EN13
title
2.2
m
total depth of water column