2024-05-14
ENA first public
ERC000021
ENA-CHECKLIST
SAMEA114579373
External Id
IOW Leibniz-Institut fur Ostseeforschung Warnemuende
INSDC center name
2023-11-06T10:23:56Z
INSDC last update
public
INSDC status
ERS16667945
SRA accession
131136:7c5a4a83-4c9c-42c9-97d6-2ddd1da6df67
Submitter Id
temperate marginal sea biome [ENVO:01000856]
broad-scale environmental context
GFBIO
broker name
2021-08-10
collection date
0-0.01
m
depth
Coastal sediment metagenome taken near Mytilus bed
description
-7.1
m
elevation
shallow marine sediment [ENVO:03000034]
environmental medium
sediment
environmental package
yes
environmental_sample
lib_3
experiment
Estonia
geographic location (country and/or sea)
58.66925
DD
geographic location (latitude)
23.06161
DD
geographic location (longitude)
Coastal samples
geographic location (region and locality)
mimarks-survey
investigation type
microbial community [PCO:1000004]
local environmental context
Samples were thawed and homogenized. A subsample of 500 mg of sediment was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit
nucleic acid extraction
marine sediment metagenome
organism
FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC
pcr primers
16S and 18S rRNA amplicon sequencing of coastal microbial communities across the salinity gradient of the Baltic Sea in pelagic, benthic, and biofilm environments
project name
B
run
Stored at -80 C
sample material processing
Coastal sediment metagenome taken near Mytilus bed
sample_description
marine sediment metagenome
scientific_name
sediment
sediment environmental package
Illumina MiSeq
sequencing method
true
submitted to insdc
V3-V4
target subfragment
28Aa
title
7.1
m
total depth of water column