2024-05-15
ENA first public
ERC000011
ENA-CHECKLIST
Swedish University of Agricultural Science, Uppsala
INSDC center name
public
INSDC status
ERS19850097
SRA accession
E-MTAB-14078:P14601_1018_S9
Submitter Id
ArrayExpress
broker name
epithelial cell
cell_type
not collected
collection date
59.78233273
collection latitude
17.62738681
collection longitude
caught in the wild
collection site
Protocols: L. sclopetarius adult female spiders were collected in the wild in a small habitat in Uppsala, Sweden. Taxonomic identity of the spider was verified by the Museum of Natural History, Stockholm, Sweden. The spiders were kept in big containers that allowed them to spin webs. They were fed with meal worms or Drosophila flies weekly and watered daily. In total 20 spiders were used for single cell sequencing.  Method A: The major ampullate glands were taken out in ringer solution, pH 7.4. The duct was removed. The glands were washed with PBS, pH 7.4 and then incubated into pre-warmed trypsin-EDTA (Gibco, 0.5%) at 37°C for 1 min in a low-binding micro-centrifuge tube. The glands were triturated with a pipette briefly and centrifuged at 300 g for 3 min at 4°C. The pellets were resuspended in 300 μL DMEM (Gibco) containing 1% BSA (Sigma). The DMEM and the tubes were briefly bubbled with carbogen prior to resuspension. The suspension was strained through a 40 μm cell strainer and transferred to low-binding micro-centrifuge tubes. The library preparation was done using 10X 3’ GE kit on the 10X Chromium Single Cell 3’ Platform following manufacturer’s protocol (Dual Index 10X_3'_V3.1).
description
adult
developmental stage
wild type genotype
genotype
Sweden
geographic location
not collected
geographic location (country and/or sea)
not applicable
individual
not applicable
isolate
Larinioides sclopetarius
organism
Larinioides sclopetarius
organism
Major ampullate gland
organism part
18
replicate
Larinioides sclopetarius
scientific_name
female
sex
Silked
silked status
not applicable
strain
P14601_1018_S9
title