F2 sporophytes derived from a bulk gametophyte cross between two strains of Saccharina latissima collected at Perharidy (near Roscoff, north Finistere, Brittany, France, January 2016, strain SLPER139) and Saint Guenole (south Finistere, Brittany, France, October 2015, strain SLSTG055) were cultivated on an open water rope system in Morlaix Bay in 2017.The F1 hybrid was strain F1.27.2. Under laboratory conditions, three small tissue disks cut from the blade of an F2 sporophyte individual were acclimatised for 4 days at 13C and then heat stressed at 21C for 7 days followed by an incubation at 13C for 16 days to allow recovery before harvesting (recovery sample). Fv/Fm measurements were carried out after heat stress and after the recovery period.
Culture conditions
RNA
DNA or RNA extracted
9
DNA or RNA quality (ratio 260/280)
2024-05-15
ENA first public
ERC000043
ENA-CHECKLIST
09/11/2023
Extraction date
Macherey-Nagel RNAplus plus 1 wash RW2
Extraction method
19/10/2017 + 17/11/2022
Harvest date
Genoscope CEA
INSDC center name
public
INSDC status
ERS19866008
SRA accession
54-R-2
Submitter Id
na
additional_details
missing: data agreement established pre-2023
collection date
sporophyte
generation
France
geographic location (country and/or sea)
missing: data agreement established pre-2023
DD
geographic location (latitude)
missing: data agreement established pre-2023
DD
geographic location (longitude)
Zofia Nehr
identification by
Morphology
identification method
Saccharina latissima
organism
diploid
ploidy
2,19
ratio 260/230
54-R-2
sample alias
RNA extracted from Saccharina latissima strain F2.27.2-54 for analysis of differential gene expression..
sample description
coastal
sampling ecosystem
Atlantic Ocean
sampling sea
Genetic cross of Perharidy and Saint Guenole strains, France
sampling site
marine
sampling water type
Saccharina latissima
scientific_name
CIT_FC
sequencing centre sample code
na
sex
na
sex determination method
F2.27.2-54
strain
blade disks
tissue type
RNA extracted from Saccharina latissima strain F2.27.2-54.
title