2024-05-15
ENA first public
ERC000011
ENA-CHECKLIST
Centre for Discovery Brain Sciences, University of Edinburgh
INSDC center name
public
INSDC status
ERS19849998
SRA accession
E-MTAB-14070:Fig1_NM_DIV8_N3
Submitter Id
E20.5
day
age
ArrayExpress
broker name
not collected
collection date
Norway rat
common name
Protocols: Primary cell cultures of cortical neurons and cortical astrocytes were generated from tissue collected from embryonic day 17.5 CD1 mice and embryonic day 20.5 Sprague-Dawley rats. RNA extraction was carried out using the High Pure RNA Tissue kit (Roche) as per manufacturer’s instructions. Barcoded RNA-seq libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturer’s instructions.
description
embryo stage
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
neuronal monoculture
growth condition
not applicable
individual
not applicable
isolate
Rattus norvegicus
organism
Rattus norvegicus
organism
brain
organism part
Rattus norvegicus
scientific_name
not applicable
sex
none
stimulus
Sprague-Dawley
strain
Fig1_NM_DIV8_N3
title