2024-05-16
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114501743
External Id
Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), 78000, Versailles, France.
INSDC center name
2023-10-16T18:18:33Z
INSDC last update
public
INSDC status
ERS16483395
SRA accession
E-MTAB-13442:MeioMeNGS_1
Submitter Id
ArrayExpress
broker name
not collected
collection date
thale cress
common name
Col-0
cultivar
Protocols: Liquid nitrogen frozen 15 days-old in vitro culture plants. Arabidopsis seeds were surface sterilized for 10 min in 70% ethanol + 0.05% sodium dodecyl sulfate and washed for 10 min in 70% ethanol and grown in Petri dishes with culture medium (Gamborg B5 medium-Duchefa supplied with 0.16% bromocresol purple and 0.1% sucrose). The generation of GFP-tagged CENH3 (GFP::CENH3) has been described in (Ravi and Chan, 2010) The generation of RFP-tagged REC8 (REC8 :RFP) line in (Kuttig et al., 2022) DNA was prepared using the NucleoSpin Plant II kit (Macherey Nagel) following the manufacturer’s instructions. Standard genomic library construction
description
rosette growth stage
developmental stage
transformant with NUP54 genomic fused with GFP
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Arabidopsis thaliana
organism
Arabidopsis thaliana
organism
rosette leaf
organism part
Arabidopsis thaliana
scientific_name
MeioMeNGS_1
title