2024-05-17
ENA first public
ERC000011
ENA-CHECKLIST
UNIVERSITY OF SZEGED
INSDC center name
public
INSDC status
ERS19893064
SRA accession
toti_minion_WGS_20230802_invitrogen_I4
Submitter Id
Top strand: 5'-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3' / Bottom strand: 5'-ACGTAACTGAACGAAGTACAGG-3'
adapters
Csongrad county
broad-scale environmental context
minitax
chimera check software
2021-12-01
collection date
dog
common name
Dog stool sample of Toti
description
no
environmental medium
Hungary
geographic location (country and/or sea)
46.253
geographic location (latitude)
20.14824
geographic location (longitude)
13 years
host age
stool
host body product
Hungarian herding terrier: Pumi
host common name
omnivore
host diet
Harsanyi-Tekergo Bator
host family relationship
Canis lupus familiaris
host scientific name
male
host sex
not applicable
host subject id
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host subspecific genetic lineage
9615
host taxid
Native Barcoding Kit 24 V14 (SQK-NBD114.24)
library construction method
not provided
library reads sequenced
ligation based
library screening strategy
not provided
library size
Szeged
local environmental context
TAGGGAAACACGATAGAATCCGAA
multiplex identifiers
LongAmp Taq 2X Master Mix
nucleic acid amplification
PureLink Microbiome DNA Purification Kit, Invitrogen
nucleic acid extraction
Canis lupus familiaris
organism
not collected
organism count
no pcr
pcr conditions
not applicable
pcr primers
Ultra-deep, hybrid metagenomic sequencing of canine gut microbiome
project name
n.a
sample storage duration
-80
sample storage temperature
Canis lupus familiaris
scientific_name
LongQC
sequence quality check
Oxford Nanopore, SQK-NBD114.24
sequencing method
whole genome
target gene
not applicable
target subfragment
20
temperature
toti_minion_WGS_20230802_invitrogen_I4
title