2024-05-17
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113550156
External Id
The First Affiliated Hospital of Kunming Medical University
INSDC center name
2023-05-25T10:57:28Z
INSDC last update
public
INSDC status
ERS15546575
SRA accession
E-MTAB-13017:Sample 29
Submitter Id
1 to 3
day
age
ArrayExpress
broker name
neuron
cell_type
house mouse
common name
Protocols: Primary cortical neuron culture was established from C57BL/6 neonatal mice aged 1-3 days by collecting brain tissue from the cerebral cortex after decapitation, and divided into control group, OGD/R group, and breviscapine administration group. Total RNA was isolated and purified by TRIzol reagent following the instruction manual. These compounds doublets of DNA and RNA were converted into DNA doublets, dUTP Solution was incorporated into the doublets at the same time, and the ends of the double-stranded DNA were complemented to flat ends. An additional base was added to each end to enable ligation with a linker with a T base at the end, and its fragment size was screened and purified using magnetic beads. The second strand was digested with UDG enzyme, and then PCR was performed to form a library with a fragment size of 300bp ± 50bp.
description
neonate
developmental stage
stroke
disease
oxygen-glucose deprivation/reoxygenation
environmental stress
primary cortical neuron culture
growth condition
Mus musculus
organism
Mus musculus
organism
brain
organism part
Mus musculus
scientific_name
C57BL/6
strain
therapy
study group
Sample 29
title