2024-05-17
ENA first public
ERC000011
ENA-CHECKLIST
UNIVERSITY OF SZEGED
INSDC center name
public
INSDC status
ERS19893069
SRA accession
BACMIX_minion_v1_9_20230623_zymo_magbead_NZ3
Submitter Id
16SFw 5' - ATCGCCTACCGTGAC - barcode - AGAGTTTGATCMTGGCTCAG - 3'; 16S Rev 5' - ATCGCCTACCGTGAC - barcode - CGGTTACCTTGTTACGACTT - 3'
adapters
not applicable
broad-scale environmental context
minitax
chimera check software
not collected
collection date
ZymoBIOMICS Microbial Community Standard
description
DNA/RNA Shield (R1100-50)
environmental medium
not applicable
geographic location (country and/or sea)
not applicable
geographic location (latitude)
not applicable
geographic location (longitude)
not applicable
host age
not applicable
host body product
not applicable
host common name
not applicable
host diet
not applicable
host family relationship
not applicable
host scientific name
not applicable
host sex
not applicable
host subject id
not applicable
host subspecific genetic lineage
not applicable
host taxid
16S Barcoding Kit (SQK-RAB204)
library construction method
not provided
library reads sequenced
ligation based
library screening strategy
not provided
library size
not applicable
local environmental context
GTTTCATCTATCGGAGGGAATGGA
multiplex identifiers
LongAmp Taq 2X Master Mix
nucleic acid amplification
ZymoBIOMICS 96 MagBead DNA Kit, Zymo
nucleic acid extraction
gut metagenome
organism
10.5x10^9
organism count
Initial denaturation 1 min 95 °C (1 cycle); Denaturation 20 secs 95 °C (25 cycles); Annealing 30 secs 55 °C (25 cycles); Extension 2 mins 65 °C (25 cycles); Final extension 5 mins 65 °C (1 cycle)
pcr conditions
27F 5'-AGAGTTTGATCCTGGCTCAG-3'; 1492R 5'-GGTTACCTTGTTACGACTT-3'
pcr primers
Ultra-deep, hybrid metagenomic sequencing of canine gut microbiome
project name
96h
sample storage duration
-80
sample storage temperature
gut metagenome
scientific_name
LongQC
sequence quality check
Oxford Nanopore, SQK-RAB204
sequencing method
16S rRNA
target gene
V1-V9
target subfragment
20
temperature
BACMIX_minion_v1_9_20230623_zymo_magbead_NZ3
title