2024-05-17
ENA first public
ERC000011
ENA-CHECKLIST
Centre for Discovery Brain Sciences, University of Edinburgh
INSDC center name
public
INSDC status
ERS19897208
SRA accession
E-MTAB-14081:20G_M-2
Submitter Id
E20.5
day
age
H3K27me3
antibody
ArrayExpress
broker name
not collected
collection date
Norway rat
common name
Protocols: Primary cell cultures of cortical neurons and cortical astrocytes were generated from tissue collected from embryonic day 17.5 CD1 mice and embryonic day 20.5 Sprague-Dawley rats. Chromatin immunoprecipitation was performed with the SimpleChIP® Plus Enzymatic ChIP Kit (Magnetic Beads) (Cell Signalling Technology, #9005) according to the manufacturer’s protocol. Library preparation for the samples were performed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs Inc, #E7645S) according to the manufacturer’s protocol.
description
embryo stage
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
coculture of rat neurons and mouse astrocytes
growth condition
not applicable
isolate
Rattus norvegicus
organism
Rattus norvegicus
organism
brain
organism part
Rattus norvegicus
scientific_name
not applicable
sex
Sprague-Dawley
strain
20G_M-2
title