2024-05-17
ENA first public
ERC000011
ENA-CHECKLIST
EMBL-EBI
INSDC center name
public
INSDC status
ERS19941419
SRA accession
E-MTAB-11267_2:WSSS_F_LNG10282020
Submitter Id
8
week
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Protocol carried out by HDBR to extract specimen from a fetal donor.  Human fetal lung organoids were derived and maintained as previously described (Nikolic et al., 2017). In brief, human foetal lung tissues were treated with Dispase (8 U/ml Thermo Fisher Scientific, 17105041) at room temperature (RT) for 2 min to digest mesenchymal connections. Mesothelium and mesenchymal cells were carefully removed by needles. Branching epithelial tips were micro-dissected by needles, transferred into Matrigel (356231, Corning) and seeded in a 24 well low-attachment plate (M9312-100EA, Greiner) with 4-5 tips per 50 μl Matrigel dome per well. The plate was incubated at 37°C for 5-10 min until the Matrigel domes solidified. 600 μl of self-renewing differentiation medium was added. Organoids were cultured under standard tissue culture conditions (37°C, 5% CO2), maintained in self-renewing medium and passaged by mechanically breaking using P200 pipettes every 4-7 days. Organoids were then fully dissociated into single cells by incubation with prewarmed TrypLE (Thermo Fisher Scientific, 12605028) for 15-20 min with trituration every 5 min.  Libraries were prepared according to the standard protocol of the Chromium 5'V1 kit.
description
Carnegie stage 22
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
organoid culture
growth condition
HDBR1915||HDBR2174||HDBR-N13393
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
male
sex
WSSS_F_LNG10282020
title