2024-05-18
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA14515671
External Id
KOTAI Biotechnologies, Inc.
INSDC center name
2022-06-06T09:54:49Z
INSDC last update
public
INSDC status
ERS12147624
SRA accession
E-MTAB-11775:1294_covid_libraseq_200811_sort_2
Submitter Id
22, 22, 36, 49, 49, 72, 72
year
age
ArrayExpress
broker name
peripheral blood mesothelial cell
cell_type
human
common name
Protocols: Blood was collected in Vacutainer CPT tubes (BD), followed by centrifugation at 1800 x g for 20min. PBMCs were washed with PBS three times and stored at -135 oC. Antigen-specific B cells were purified by staining with fluorescent-labeled DNA-barcoded antigen and antibodies using FACS. Single-cell suspensions of sorted B cells were loaded onto the Chromium Controller microfluidics device (10x Genomics) and processed using the B cell Single Cell V(D)J solution according to the manufacturer’s protocol. For amplify and purify the antigen barcode libraries, 10x Genomics single-cell VDJ human B cell assay and target enrichment protocol were completed.
description
COVID-19
disease
convalescence
disease staging
pool3_male x 3 female x 4
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
mixed male and female
sex
1294_covid_libraseq_200811_sort_2
title