2024-05-21
ENA first public
ERC000011
ENA-CHECKLIST
MPUSP
INSDC center name
public
INSDC status
ERS18335001
SRA accession
EC3741
Submitter Id
ErmR
antibiotic_resistance
Max Planck Unit for the Science of Pathogens
collected_by
2023-10-11
collection date
THY
culture_medium
S. pyogenes SF370 was transformed with plasmid p7INT.1, and transformants were selected on TSA plates with 5% sheep blood and 3 �g/mL erythromycin. A single colony was grown in THY without antibiotic and flash-frozen in liquid nitrogen in THY with 20% glycerol. The strain was then streaked on a TSA plate with 5% sheep blood, grown overnight in THY and finally grown to early stationary growth phase in fresh THY medium. Cells were pelleted, washed with PBS and stored at -80 degree C.
description
attB(T12)::p7INT.1
genotype
Germany
geographic location (country and/or sea)
Streptococcus pyogenes M1 GAS
organism
Streptococcus pyogenes M1 GAS
scientific_name
M1
serotype (required for a seropositive sample)
SF370
strain
S. pyogenes SF370 transformed with p7INT.1, clone 1
title