2024-05-21
ENA first public
ERC000011
ENA-CHECKLIST
Wellcome Sanger Institute
INSDC center name
public
INSDC status
ERS20065162
SRA accession
E-MTAB-11640_2:WSA_LngSP10972074_imaged
Submitter Id
70 to 74
year
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Samples no larger than 0.5cm2 were cut from each of the 5 locations of lung (trachea, bronchi at the 2nd/3rd generation, bronchi at the 4th generation, upper left lobe parenchyma and lower left lobe parenchyma) and additional small airway. Majority of the parenchyma was removed from the airway tissues, embedded in OCT and flash frozen in dry-ice cooled isopentane at -55°C.  Haematoxylin and eosin staining was used to determine the morphology of tissue blocks, in order to choose those to proceed to Visium. Ten micron sections were then cut from the blocks onto Visium slides (10X Genomics) and processed according to the manufacturer’s protocol. Nucleic acid was extracted according to the standard protocol of the Spatial 3' v1 protocol Tissue sections were loaded onto Visium chips according to the standard protocol of the Spatial 3' v1 protocol. Dual-indexed libraries were prepared, pooled at 2.25nM and sequenced.
description
adult
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
A61
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
trachea
organism part
Homo sapiens
scientific_name
female
sex
WSA_LngSP10972074_imaged
title