2024-05-21
ENA first public
ERC000011
ENA-CHECKLIST
Gurdon Institute, University of Cambridge
INSDC center name
public
INSDC status
ERS20099002
SRA accession
E-MTAB-14100:EMS23
Submitter Id
ArrayExpress
broker name
not collected
collection date
fruit fly
common name
Protocols: To extract genomic DNA for sequencing, 10 adult males were homogenised in 500 μl of solution A buffer (0.1 M Tris-Hcl pH 7.5, 0.01 M EDTA pH 8.0, 1% SDS) and incubated for 20 min at 70°C. 70μl of 8M potassium acetate was then added to the samples and incubated for 20 min on ice. After the incubation, the samples were centrifuged for 15 min at maximum speed.  The supernatant was mixed with 250 μl of isopropanol and centrifuged for 4 min at 13,000 g. The pellet was washed with 70% ethanol and diluted in 100 μl ddH2O.  The DNA library was prepared using the Illumina DNA Prep kit (Illumina, 20018705) and index kit (Illumina, 20027213) following the manufacturer’s instructions.
description
adult
developmental stage
EMS-23
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Drosophila melanogaster
organism
Drosophila melanogaster
organism
whole organism
organism part
Drosophila melanogaster
scientific_name
male
sex
EMS23
title