2024-05-22
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114295452
External Id
University of Veterinary Medicine Vienna Institute of Pharmacology and Toxicology
INSDC center name
2023-08-22T15:33:30Z
INSDC last update
public
INSDC status
ERS16284561
SRA accession
E-MTAB-13268:Palbociclib
Submitter Id
8
week
age
ArrayExpress
broker name
hematopoietic stem cell
cell_type
not collected
collection date
house mouse
common name
Protocols: Bone marrow cells of three wild type C57BL/6N mice were isolated. After lineage depletion, cells were pooled and distributed to either PBS or Palbociclib treatment. After 24h of cultivation, suspension cells were harvested and 67700 lineage-negative, Sca1-positive, cKit-positive (LSK) cells were sorted per sample by means of flow cytometry. Cells were treated for 24h with 500nM Palbociclib or with PBS. Nucleic acid extraction was performed as part of the library construction. Libraries were generated using the Chromium NextGem Single Cell 5’ v2 Kit solution (10X Genomics), according to the manufacturer’s instructions
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
Kit positive, Sca1-positive
immunophenotype
1036-3141-43
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
bone marrow
organism part
Mus musculus
scientific_name
male
sex
C57BL/6N
strain
Palbociclib
title