2024-05-23
ENA first public
ERC000011
ENA-CHECKLIST
EMBL-EBI
INSDC center name
public
INSDC status
ERS20107616
SRA accession
E-MTAB-11278_2:SIGAC10_CS_3'v2
Submitter Id
20
week
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Protocol carried out by HDBR to extract specimen from a fetal donor.  Nucleic acid was extracted according to the standard protocol of the Chromium single cell 3’ V2 kit. Libraries were prepared according to the standard protocol of the Chromium single cell 3’ V2 kit. V(D)J libraries were prepared according to the manufacturer’s protocol (10X Genomics) using individual Chromium i7 Sample Indices.5,000 PE reads per cell for V(D)J libraries were aimed for sequencing. Libraries for gamma/delta TCR variable regions were amplified as previously described (Mimitou et al. 2019)
description
20th week post-fertilization human stage
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
HDBRL13930
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
lung
organism part
10x 3' v2
protocol
whole lung
sampling site
Homo sapiens
scientific_name
female
sex
SIGAC10_CS_3'v2
title