2024-05-24
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113902848
External Id
EMBL-EBI
INSDC center name
2023-06-20T13:41:40Z
INSDC last update
public
INSDC status
ERS15897017
SRA accession
E-MTAB-13064_5:S22855_feature
Submitter Id
ArrayExpress
broker name
SUM159PT
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: Single cell suspensions (scRNA seq) or single nuclei suspensions (Multiome) were obtained according to manufacturer’s instructions (10X Genomics). scRNA-seq and Multiome (ATAC + gene expression). Library preparation was performed according to the manufacturer’s instructions (single cell 3’ v2.1 or v3 protocol, Multiome 10x Genomics). First, reverse transcription was performed in droplet and library construction was performed, according to the 10X Chromium Single Cell 3' Reagent Kit User Guide Rev C. From the same cDNA of the total RNA library, additional libraries were obtained for GBC samples. This was performed in order to increase the reads of the specific barcodes. GBC libraries were prepared a dial-out PCR strategy to amplify the BFP 3’UTR region containing the barcode, as in Dixit A et al. DOI 10.1016/j.cell.2016.11.038.
description
adult
developmental stage
triple-negative breast cancer
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
breast
organism part
Homo sapiens
scientific_name
S22855_feature
title