Plant
BioSampleModel
2016-03-31T01:28:05Z
ENA-FIRST-PUBLIC
2016-03-31T01:28:05Z
ENA-LAST-UPDATE
SAMN04590510
External Id
pda|ammorse@ufl.edu
INSDC center name
2016-03-31T01:28:05Z
INSDC first public
2016-03-31T01:28:05Z
INSDC last update
killed
INSDC status
SRS1368034
SRA accession
CCJ
Submitter Id
8 weeks
age
Koen J.F. Verhoeven Terrestrial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, Netherlands
biomaterial_provider
NCBI
broker name
Parental lineage treatments: Control treatments were applied to the upper surface of 3-4 fully expanded leaves. In generation 1, 0.75 ml of solution per plant was applied to 8-week old plants; in generation 2, a total amount of 0.75 ml was applied to each plant distributed over two application moments when plants were 5 and 7 weeks old. In both generations treatment application was during vegetative growth, approximately 1 month prior to first flowering. Offspring treatments: Seeds from a single seed head were weighed individually, surface-sterilized and germinated on 0.8% agar plates. After 10 days, seedlings were transplanted to individual pots and grown under climate chamber conditions in fully randomized blocks. Each block contained two plants per parental lineage; one received a JA treatment and the other a control treatment. Jasmonic Acid was applied to 8-week old plants by distributing 0.25 ml of a 10 mM JA solution over the surface of two standardized leaves. 3h after JA-application two standardized leaves (younger than the JA-treated leaves) were collected, discarding the latex-rich mid-vein, flash-frozen in liquid nitrogen and stored at -80C for subsequent RNA analysis.
description
Netherlands: near Heteren
geo_loc_name
14h light / 10h dark 20C / 15C
growth_protocol
A68
isolate
Taraxacum officinale
organism
Taraxacum officinale
scientific_name
leaf
tissue
control parental treatment / JA offspring treatment
treatment