2024-05-29
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115057548
External Id
Philipps University Marburg, Bioinformatics Core Facility
INSDC center name
2023-12-22T12:55:16Z
INSDC last update
public
INSDC status
ERS17598044
SRA accession
E-MTAB-13668:MA157
Submitter Id
8 to 20
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Cell culture dishes were scrapped - see growth protocol Bone marrow was collected from femur bones of C57Bl/6 mice by flushing with RPMI-1640 medium containing 1% penicillin/streptomycin and passing through a 70-micron cell filter. The cell suspension was centrifuged at 300 g for 5 min, and the cell pellet was incubated in erythrocyte lysis buffer (155 mM NH4Cl, 12 mM NaHCO3, and 0.1 mM EDTA) for 3 min at room temperature, followed by centrifugation at 300 g for 5 min. Bone marrow cells were plated on a 6-well plate and cultured in RPMI-1640 medium (with glutamine and pyruvate) supplemented with 10% FBS, 1% penicillin/streptomycin, and 20 ng/ml of recombinant macrophage colony-stimulating factor M-CSF (Immunotools, Germany). Cells were incubated for 5 days at 37°C in a humid atmosphere containing 5% CO2 to allow differentiation into macrophages. Differentiated BMDM were treated with 1/3 v.v. of SN from tumor cells for 24 h. Control wells (untreated control) were supplemented with 1/3 v.v. of DMEM with 0.5% FBS. RNA prep with Macherey-Nagel spin columns incl on-column DNase digest “QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina” (Lexogen, Vienna, Austria)
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
pool #1
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
bone marrow
organism part
Mus musculus
scientific_name
mixed
sex
C57BL/6
strain
MA157
title