2024-05-29
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115057537
External Id
Philipps University Marburg, Bioinformatics Core Facility
INSDC center name
2023-12-22T12:54:02Z
INSDC last update
public
INSDC status
ERS17598033
SRA accession
E-MTAB-13667:MA129
Submitter Id
6 to 7
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: At the experimental endpoint mice were euthanized and tumors were removed. At the experimental endpoint mice were euthanized and tumors were removed. The study was approved by the regional agency on animal experimentation (Regierungspräsidium Giessen). C57BL/6 mice were randomly divided into two groups. 1 × 105 WT or Dyrk1b-KO (clone 5.3) mKpc4 cells were suspended in a total volume of 150 μl of DMEM medium. The cell suspensions were injected s.c. at the posterior flank of the mice. Size of tumors were measured twice per week using a caliper. Tumor volumes were calculated by the formula (length x width2)/2. The length represents the longer axis and the width represents the shorter axis of the tumor. RNA prep with Macherey-Nagel spin columns incl on-column DNase digest “QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina” (Lexogen, Vienna, Austria)
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
4
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
s.c. tumor
organism part
Mus musculus
scientific_name
female
sex
C57BL/6
strain
MA129
title