2024-05-30
ENA first public
ERC000011
ENA-CHECKLIST
Institute for Immunology and Hematology University of Strasbourg France
INSDC center name
public
INSDC status
sh TRAIL
RNA interference
ERS20065181
SRA accession
E-MTAB-14089:LigneesW-neg-T3
Submitter Id
3
month
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Grafted breast tumor tissue was collected as described previously (doi: 10.15252/emmm.202013270). Tumor samples were fresh frozen Total RNA was extracted using RNeasy® Plus Micro Kit (Qiagen) according to the manufacturer’s instructions. Libraries were prepared from RNA using SMARTer Stranded Total RNA-Seq Kit, Pico Input Mammalian (Takara) following the manufacturer’s instructions. Briefly, random primers were used for first-strand synthesis, and ribosomal cDNA was cleaved by ZapR V.2 in the presence of mammalian R-probes V.2.
description
adult
developmental stage
Tenascin KO
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Tumor
organism part
Mus musculus
scientific_name
female
sex
FVB
strain
LigneesW-neg-T3
title