2024-05-31
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA111520023
External Id
Department of Biological Sciences University of Bergen, Bergen, Norway
INSDC center name
2022-10-26T17:03:34Z
INSDC last update
public
INSDC status
ERS13630220
SRA accession
E-MTAB-12338:Mixture 4
Submitter Id
Norwegian coastal
breed
ArrayExpress
broker name
Atlantic cod
common name
Protocols: Juvenile male Atlantic cod from a strain of Norwegian coastal cod were used in this experiment. Farmed cod with mean weight 0.84±0.18 kg was transferred to 20 m3 indoor tanks prior to the start of the exposure experiment. Indoor light followed the natural diurnal rhythm for Bergen (64°N). The fish tanks were supplied with seawater at 8.8-9.5°. The fish were fed once per week (by tube-feeding) feed pellets with vehicle (control), chlorinated compounds (PCBs, DDTs chlordane, lindane and toxaphene), Brominated compounds (PBDE), fluorinated compunds (PFOS) and mixture of the chlorinated, brominated and fluorinated compounds, as detailed in Bratberg et al., 2013. Tissue sampling was conducted one week after the administration of the final dose. The cod were anaesthetized with metomidate hydrochloride and killed by a blow to the head. Liver samples were rapidly excised, snap frozen in liquid nitrogen and stored at -80°C before further processing. Liver tissue was homogenized with a Precellys 24 homogenizer (Bertin Technologies, Montigny-le-Bretonneux, France). Total RNA was extracted using the BioRobot EZ1 and RNA Tissue Mini Kit (Qiagen, Hilden, Germany) and treated with DNase. The RNA 6000 Nano LabChip kit (Agilent Technologies, Palo Alto, CA, USA) was used to evaluate the RNA integrity of the samples. The RNA integrity number (RIN) was 9.9 ± 0.2 (mean ± st.dev., n=47). Illumina HiSeq 4000 system
description
juvenile stage
developmental stage
normal
disease
wild type genotype
genotype
not applicable
isolate
Gadus morhua
organism
Gadus morhua
organism
liver
organism part
Gadus morhua
scientific_name
male
sex
Mixture 4
title