2024-06-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA110290381
External Id
Klinik und Poliklinik fur Innere Medizin II Klinikum rechts der Isar der Technischen Universitat Munchen
INSDC center name
2022-07-08T13:02:11Z
INSDC last update
public
INSDC status
ERS12387989
SRA accession
E-MTAB-11913:Sample 15
Submitter Id
5
week
age
ArrayExpress
broker name
acinar cell
cell_type
house mouse
common name
Protocols: Explanted acinar cells were collected directly after isolation (t=0 days) or after 2-3 days embedded in collagen. Collagen embedded samples were digested with collagenase for 10 min before RNA extraction. Maxwell 16 Tissue LEV Total RNA Purification Kit was used for RNA extraction according to the manufacturer's instructions (Promega) Library preparation for bulk 3’-sequencing of poly(A)-RNA was done as described previously (Parekh et al., 2016) barcoded cDNA of each sample was generated with a Maxima RT polymerase (Thermo Fisher) using oligo-dT primer containing barcodes, unique molecular identifiers (UMIs) and an adapter. 5’ ends of the cDNAs were extended by a template switch oligo (TSO) and after pooling of all samples full-length cDNA was amplified with primers binding to the TSO-site and the adapter. cDNA was tagmented with the Nextera XT kit (Illumina) and 3’-end-fragments finally amplified using primers with Illumina P5 and P7 overhangs.
description
wild type genotype
genotype
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
pancreas
organism part
Mus musculus
scientific_name
medium
stimulus
C57BL6 / FVB/129
strain
Sample 15
title