2024-06-01
ENA first public
ERC000011
ENA-CHECKLIST
Swedish University of Agricultural Science, Uppsala
INSDC center name
public
INSDC status
ERS19897222
SRA accession
E-MTAB-14082:P22555_1001_P18163_V10A21-050_B1
Submitter Id
ArrayExpress
broker name
mixed
cell_type
not collected
collection date
59.78233273
collection latitude
17.62738681
collection longitude
Protocols: L. sclopetarius adult female spiders were collected in the wild in a small habitat in Uppsala, Sweden. Taxonomic identity of the spider was verified by the Museum of Natural History, Stockholm, Sweden. The spiders were kept in big containers that allowed them to spin webs. They were fed with meal worms or Drosophila flies weekly and watered daily. Whole opisthosomas of spiders were flash-frozen in OCT medium on an isopentane-dry ice bath and samples were stored at –80°C until use. The samples were sectioned in a cryotome (10 μm) with knife temperature set at –23°C and sample holder temperature at –10°C. Eight sections from five individual spiders were carefully mounted on the capture areas (6.5 x 6.5 mm) of the Visium spatial slide (10X genomics) and permeabilized for 30 minutes following the 10X Visium spatial tissue optimization protocol.  The libraries were constructed according to Visium spatial gene expression protocol (10X genomics). For cDNA amplification 13-16 PCR cycles were performed and for the indexing 13 PCR cycles were used.
description
adult
developmental stage
Sweden
environmental history
Skarholmen, Uppsala
environmental history
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
individual
not applicable
isolate
Larinioides sclopetarius
organism
Larinioides sclopetarius
organism
Whole abdome (opsthosoma) sections
organism part
6
replicate
caught in the wild
sampling site
Larinioides sclopetarius
scientific_name
female
sex
P22555_1001_P18163_V10A21-050_B1
title