2024-06-01
ENA first public
ERC000011
ENA-CHECKLIST
School of Medicine, Cardiff University, UK UK Dementia Research Institute, Cardiff University, Cardiff
INSDC center name
public
INSDC status
ERS19897198
SRA accession
E-MTAB-14080:B4_Sl_knockdown
Submitter Id
14
day
age
ArrayExpress
broker name
not collected
collection date
fruit fly
common name
Protocols: 10 heads from 14 day old Drosophila melanogaster were dissected and pooled per replicate, per genotype. Tissue was snap frozen prior to extraction. Pooled heads were manually homogenised and RNA was subsequently extracted using the RNAqueous-Micro kit (AM1931) as per the manufacturer's instructions. The Cardiff University Genome Hub performed Qubit and Tapestation quality/quantity controls prior to library preparation. Samples were then normalised and library prep was performed. The latter was achieved following the Illumina TruSeq stranded mRNA kit. Here mRNA was enriched by poly-A selection and Truseq3 adapters added.
description
adult
developmental stage
Repo-GAL4/UAS-Sl RNAi BDSC 32906
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Drosophila melanogaster
organism
Drosophila melanogaster
organism
head
organism part
Drosophila melanogaster
scientific_name
female
sex
B4_Sl_knockdown
title