2024-06-04
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113601390
External Id
SC
INSDC center name
2023-06-15T10:12:43Z
INSDC last update
public
INSDC status
ERS15595771
SRA accession
E-MTAB-13065:FCAImmP7812662
Submitter Id
15
week
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Samples were collected by the Human Developmental Biology Resource and dissected into different organs. Skin samples were enzymatically digested using collagenase to produce a single cell suspension. Cells were FACS sorted, live, CD45 positive fraction was loaded onto the 10x Chromium using the manufacturer's protocols. During cell partitioning, cells are lysed to release RNA molecules that bind to 10x's proprietary gel beads to barcode transcripts. Barcoded RNA molecules were reverse transcribed into cDNA and nested PCR reactions were performed to enrich for the specific T cell receptor cDNA. Libraries were generated using the 10x Genomics protocols for their Single Cell Immune Receptor Profiling kits.
description
fetus
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
CD45 positive
immunophenotype
F50
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
skin
organism part
Homo sapiens
scientific_name
female
sex
F50_SK_CD45P_2
title
FCAImmP7812662
title