2024-06-04
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113565806
External Id
Wellcome Sanger Institute
INSDC center name
2023-05-31T11:49:02Z
INSDC last update
public
INSDC status
ERS15561954
SRA accession
E-MTAB-13024:HCA_rFSKI13460603
Submitter Id
10
week
age
ArrayExpress
broker name
human
common name
Protocols: Fetal tissue samples were collected at the Cambridge University Hospitals NHS Foundation Trust under NHS REC 96/085. Fetal facial and abdominal skin samples from a single donor at 10 post conception weeks (PCW) were embedded in optimal cutting temperature (OCT) medium and flash-frozen in isopentane cooled with dry-ice. Sections were chosen based on prior morphology (H&E) and RNA quality checks. 10µm cryosections from the OCT blocks were cut onto 10x Genomics Visium slides. Sections were stained with hematoxylin and eosin (H&E), imaged and processed according to the 10x Genomics Visium protocol, using a permeabilization time of 12 min found through a prior tissue optimization step. After transcript capture, dual-indexed libraries were prepared as per manufacturer’s protocol.
description
embryo
developmental stage
normal
disease
BRC2318
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
skin of abdomen
organism part
Homo sapiens
scientific_name
HCA_rFSKI13460603
title