2024-06-05
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA14510799
External Id
KAROLINSKA INSTITUTET
INSDC center name
2022-06-05T12:44:34Z
INSDC last update
public
INSDC status
ERS12142759
SRA accession
V4_1_in_1000_synthetic_lib5_Plate1_D4
Submitter Id
malaria parasite P. falciparum
common name
To assess the specificity and sensitivity of sequencing and variant calling, the genes encoding msp2 of the P. falciparum reference strains 3D7 (IC1 family, size variant of 473bp), HB3 (FC27 family, size variant of 292bp), CD01 (FC27 family, size variant of 292bp), Dd2 (FC27 family, size variant of 412bp), SN01 (FC27 family, size variant of 292bp), KE01 (FC27 family, size variant of 376bp), SD01 (IC1 family, size variant of 434bp), GN01 (IC1 family, size variant of 539bp), 7G8 (IC1 family, size variant of 485bp) and GB4 (IC1 family, size variant of 533bp) were chemically synthesized (GeneScript) and inserted into the pUC57 vector by EcoRV (GAT | ATC) blunt ligation. Multiclonal mock infections were prepared by diluting different molar ratios of the variants in water in complexities of infection ranging from 1 to 10. Each mock infection was assigned to a unique barcode combination
description
Blood_stage
dev_stage
Homo_sapiens
host scientific name
Blood_cell_pellets
isolation_source
Plasmodium falciparum
organism
Plasmodium falciparum
scientific_name
V4_1_in_1000_synthetic_lib5_Plate1_D4.fastq
title