2024-06-05
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114316758
External Id
UOM
INSDC center name
2023-08-30T15:23:22Z
INSDC last update
public
INSDC status
ERS16305872
SRA accession
E-MTAB-13317:PD193 ZT4 N
Submitter Id
13 to 17
week
age
ArrayExpress
broker name
fibroblast-like synoviocyte
cell_type
not collected
collection date
house mouse
common name
Protocols: Paws were dissected to remove skin and separate joints, then incubated with collagenase IV (10 mg/ml, Sigma C5138) in DMEM containing 10% FBS and 1% penicillin-streptomycin at 37ºC with shaking for 1-1.5 hours. FLS cells were selected using the EasySep Release Mouse PE Positive Selection kit (Stemcell, 17656) and a Podoplanin-PE conjugated antibody (BioLegend 127407). FLS cell pellets were lysed in buffer RLT Plus supplemented with DTT, and RNA isolated using the RNeasy Micro Plus kit (Qiagen) according to the manufacturer’s instructions. Sequencing libraries were generated using the Stranded mRNA Prep Ligation kit (Illumina, Inc) according to the manufacturer’s protocol. Library fragment size and quality was assayed using a 4200 TapeStation (Agilent Technologies).
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
hindlimb joint
organism part
Mus musculus
scientific_name
male
sex
DBA1
strain
PD193 ZT4 N
title