SAMD00018756
External Id
KYOTO-SC
INSDC center name
2015-02-23T00:00:00Z
INSDC first public
2024-06-02T01:40:32.653Z
INSDC last update
DRS019023
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
DRS019023
SRA accession
Total RNA and polyA+ RNA were extracted from pools, each of which contained one hundred MII oocytes or 2-cell embryos (C57BL/6 X ICR), by using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). Four replicates were made for directional RNA-seq library construction by using an NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB). In this library preparation, cDNAs were enriched by 15-cycle PCR. Illumina HiSeq 2000 was used to perform 50-bp single-end sequencing according to the manufacturerÂfs instructions.
description
MII oocyte
host body site
Dynabeads
isolate
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
MII_3
sample name
C57BL/6 X ICR
strain
MII_3
title