SAMN05860096
External Id
NIAID Integrated Research Facility
INSDC center name
2016-10-03T00:00:00Z
INSDC first public
2017-04-12T21:00:56.153Z
INSDC last update
live
INSDC status
Pathogen.cl
NCBI submission model
Pathogen.cl.1.0
NCBI submission package
ERS20229712
SRA accession
1994
collection date
This Master stock was propagated from founder material orginally produced at USAMRIID. VERO C1008 (E6) cells, Kidney (African green monkey), Working Cell Bank, NR-596 were obtained through BEI Resources (National Institute of Allergy and Infectious diseases [NIAID], National Institutes of Health [NIH], Manassas, VA). Virus stock was propagated using Minimum Essential Medium-alpha, GlutaMAX, no nucleosides (Gibco, ThermoFisher Scientific) supplemented with 2% US-origin, certified, heat-inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific). Following harvest, HI-FBS was QS'd to 10% final concentration prior to cryopreservation.
description
TrizolLS
extraction method
Australia: Hendra
geo loc name
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
host
HeV disease
host disease
IRF0167
isolate
Vero C1008 (E6) cells
lab-host
VERO C1008 (E6) cells
laboratory host
Henipavirus hendraense
organism
harvest date: 14-May-2015 passaged 6x in cell culture
orgmod_note
11-May-2015 to 14-May-2015
passage date
CRFK+4, Vero76+1, VeroE6+1
passage_history
Integrated Research Facility (Honko, Anna N.; Sword, Jennifer M.)
stock production
Hendra virus (p tissue culture Master Stock, USAMRIID stock)
title