SAMN05859702
External Id
NIAID Integrated Research Facility
INSDC center name
2016-10-03T00:00:00Z
INSDC first public
2021-02-24T10:24:10.470Z
INSDC last update
live
INSDC status
Pathogen.cl
NCBI submission model
Pathogen.cl.1.0
NCBI submission package
ERS20229710
SRA accession
Centers for Disease Control and Prevention
collected by
2005-03-13
collection date
The starting material for Marburg Angola virus (full designation: Marburg virus/H.sapiens-tc/AGO/2005/Ang-1379v) was obtained from Dr. Thomas Ksiazek (University of Texas Medical Branch at Galveston, Galveston, TX, USA). VERO C1008 (E6) cells, Kidney (African green monkey), Working Cell Bank, NR-596 were obtained through BEI Resources (National Institute of Allergy and Infectious diseases [NIAID], National Institutes of Health [NIH], Manassas, VA). The IRF0169_MARV Master stock was propagated using Minimum Essential Medium-alpha, GlutaMAX, no nucleosides (Gibco, ThermoFisher Scientific) supplemented with 2% US-origin, certified, heat-inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific). Following harvest, HI-FBS was QS'd to 10% final concentration prior to cryopreservation.
description
TrizolLS
extraction method
Angola
geo loc name
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
host
8 Mo
host age
Marburg Virus Disease
host disease
female
http://purl.obolibrary.org/obo/PATO_0000383
host sex
810838
host subject id
Fatal case
host_disease_outcome
IRF0169
isolate
serum
http://purl.obolibrary.org/obo/BTO_0000133
isolation source
Vero C1008 (E6) cells
lab-host
VERO C1008 (E6) cells
laboratory host
Orthomarburgvirus marburgense
organism
harvest date: 12-Jun-2015; passaged 3x in cell culture
orgmod_note
05-Jun-2015 to 12-Jun-2015
passage date
VeroE6+3
http://purl.obolibrary.org/obo/CLO_0009524
passage_history
Integrated Research Facility (Cai, Yingyun; Sword, Jennifer M.; Honko, Anna N.)
stock production
Marburg virus Angola (p3 tissue culture Master Stock, UTMB stock)
title