SAMN05860095
External Id
NIAID Integrated Research Facility
INSDC center name
2016-10-03T00:00:00Z
INSDC first public
2017-04-12T21:00:59.780Z
INSDC last update
live
INSDC status
Pathogen.cl
NCBI submission model
Pathogen.cl.1.0
NCBI submission package
ERS20229711
SRA accession
Centers for Disease Control and Prevention
collected by
1999
collection date
This Master stock was propagated from founder material orginally at USAMRIID [label read: E6 p3 3/24/05, Fr: NIVp2, 7-03-03, 1:10 (assume SPB19901924)]. VERO C1008 (E6) cells, Kidney (African green monkey), Working Cell Bank, NR-596 were obtained through BEI Resources (National Institute of Allergy and Infectious diseases [NIAID], National Institutes of Health [NIH], Manassas, VA). Virus stock was propagated using Minimum Essential Medium-alpha, GlutaMAX, no nucleosides (Gibco, ThermoFisher Scientific) supplemented with 2% US-origin, certified, heat-inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific). Following harvest, HI-FBS was QS'd to 10% final concentration prior to cryopreservation.
description
TrizolLS
extraction method
Malaysia: Kuala Lumpur
geo loc name
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
host
NiV disease
host disease
male
http://purl.obolibrary.org/obo/PATO_0000384
host sex
Fatal case
host_disease_outcome
IRF0158
isolate
Vero C1008 (E6) cells
lab-host
VERO C1008 (E6) cells
laboratory host
Henipavirus nipahense
organism
harvest date: 23-Mar-2015; passaged 4x in cell culture
orgmod_note
20-Mar-2015 to 23-Mar-2015
http://www.ebi.ac.uk/efo/EFO_0008796
passage date
Vero76+1, VeroE6+3
http://purl.obolibrary.org/obo/CLO_0009524
passage_history
Integrated Research Facility (Honko, Anna N.; Sword, Jennifer M.)
stock production
Nipah virus Malaysia (p4 tissue culture Master Stock, USAMRIID stock)
title