2024-06-12
ENA first public
ERC000024
ENA-CHECKLIST
SAMEA115158254
External Id
IOW Leibniz-Institut fur Ostseeforschung Warnemuende
INSDC center name
2024-01-26T15:39:55Z
INSDC last update
public
INSDC status
ERS17891065
SRA accession
137854:04dc8a2c-fe73-476e-88e6-5307fb8c3e7d
Submitter Id
aquatic biome [ENVO:00002030]
broad-scale environmental context
GFBIO
broker name
2021-06-08
collection date
sequences were generated in mixed orientation, separate read files submitted for each orientation (here: R1 file contains forward reads)
comment
1.5
m
depth
water sample collected on day1 from mesocosm number 2 nitrogen addition
description
brackish water [ENVO:00002019]
environmental medium
water
environmental package
yes
environmental_sample
Baltic Sea
geographic location (country and/or sea)
59.84
DD
geographic location (latitude)
23.24
DD
geographic location (longitude)
Tvaerminne, Finland
geographic location (region and locality)
mimarks-survey
investigation type
microbial community [PCO:1000004]
local environmental context
doi:10.1128/AEM.71.11.6885-6899.2005
nucleic acid extraction
aquatic metagenome
organism
FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAAKCC
pcr primers
INN:PP AQUACOSM
project name
filtration of 500 ml of water sample on two different sterile polycarbonate filters with pore size of 0.2 micrometers before immediate flash-freezing in liquid nitrogen and storage at -80 celsius degrees until nucleic acid extraction
sample material processing
water sample collected on day1 from mesocosm number 2 nitrogen addition
sample_description
aquatic metagenome
scientific_name
Illumina MiSeq
sequencing method
true
submitted to insdc
V3-V4
target subfragment
1point2
title
water
water environmental package