2024-06-15
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA110132318
External Id
Pavel Pavlov
INSDC center name
2022-06-15T15:19:41Z
INSDC last update
public
INSDC status
ERS12231138
SRA accession
HFD-53_P24807_112
Submitter Id
Lisha Wang, Jakub Wojcieszak
collected_by
2021-10-15
collection_date
house mouse
common name
We use the high-fat diet (HFD) to build obese model in mice and evaluate effects of our FKBP51-Hsp90 interaction inhibitor (E8). There are four groups: Control diet (CD), HFD, CD + E8, and HFD+E8. We isolated total RNA from the hippocampus (n=5/group, 20 in total) using RNeasy Lipid Tissue Mini Kit (Cat. No. 74804, Qiagen, Germany) according to its handbook. RNA concentration and quality were assessed using fluorometric measurements on the Qubit 3.0 instrument (ThermoFisher Scientific) and capillary electrophoresis (Caliper GX II, Agilent), respectively. Sample ID in four groups: HFD+E8 (P24807_101 to P24807_105); CD+E8 (P24807_106 to P24807_110); HFD+Vehicle (P24807_111 to P24807_115); CD+Vehicle (P24807_116 to P24807_120).
description
Sweden
geographic location (country and/or sea)
Solna
geographic location (region and locality)
Hippocampus
isolation_source
Janvier Labs
lab_host
Mus musculus
organism
Mus musculus
scientific_name
male
sex
C57BL/6J mice
strain
Mouse hippocampus
tissue_type
High-fat diet and Vehicle (HFD+Vehicle)
title