2024-06-18
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114237552
External Id
Institute of Oncology research (IOR)
INSDC center name
2023-08-04T14:23:56Z
INSDC last update
public
INSDC status
ERS16223002
SRA accession
E-MTAB-13229:cEHFko_6
Submitter Id
24
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: GEMM prostate glands were harvested and snap frozen in RNase-free conditions. Total RNA was extracted from GEMM prostates as described in following details. Briefly, the entire mouse prostates were disrupted with pestle (Axygen PES-15-B-SI Disposable Tissue Grinder Pestle) in 1.5 ml Eppendorf tubes loaded with 350 µl of RLT buffer (RNeasy® Mini Kit, Qiagen, cat. Numb. 74104). RNA was extracted by following RNeasy® Mini Kit instructions. The RNA quality was verified by the spectrophotometric analysis by using 2100 Bioanalyzer System (Agilent, cat. Numb. G2939BA). Only samples with R.I.N. (RNA integrity number) 8-10 were used for Poly-A RNA sequencing analysis. NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was employed with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences.
description
cEHF knockout
genotype
not collected
geographic location (country and/or sea)
Replicate_6
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
prostate gland
organism part
Mus musculus
scientific_name
male
sex
cEHFko_6
title