2024-06-18
ENA first public
ERC000011
ENA-CHECKLIST
Gyeongsang National University Animal Genomics Laboratory
INSDC center name
public
INSDC status
ERS18535975
SRA accession
E-MTAB-13924:NT-4
Submitter Id
4
month
age
ArrayExpress
broker name
blood cell
cell_type
not collected
collection date
dog
common name
Protocols: Two cloned dogs(NT-2, NT-4) were produced via SCNT using ear fibroblasts from a 5-year-old male German shepherd. For whole genome sequencing, blood and ear fibroblasts were collected from a 6-year-old male German shepherd dog, and blood samples were collected from  4-month-old cloned dogs. Experiments were authorized by the Animal Center for Biomedical Experimentation at the National Institute of Animal Science of the Rural Development Administration (approval number 2015-143 on 21 May 2015). Genomic DNA was extracted from the blood of cloned dogs and original dog with a Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) and from original dog cells with PureLink™ Genomic DNA Mini Kit (Thermo Fisher Scientific, Carlsbad, CA, USA). Individual libraries were generated TruSeq Nano DNA Sample Prep Kit (Illumina, San Diego, CA, USA).
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Canis lupus familiaris
organism
Canis lupus familiaris
organism
blood
organism part
cloned dog
phenotype
Canis lupus familiaris
scientific_name
male
sex
NT-4
title