2024-06-19
ENA first public
ERC000011
ENA-CHECKLIST
Dipartimento di Medicina Molecolare e Biotecnologie Mediche (DMMBM), Università Federico II di Napoli, CEINGE Biotecnologie Avanzate Franco Salvatore
INSDC center name
public
INSDC status
ERS18459056
SRA accession
E-MTAB-13918:HEK293T_Solution3_2
Submitter Id
ArrayExpress
broker name
HEK293T
cell_line
epithelial-like cell from human kidney
cell_type
not collected
collection date
human
common name
Protocols: HEK-293T cells were treated with a nutraceutical formula containing propolis, Verbascum, thymus, long chain length polyphosphates, potassium sorbate and sodium benzoate (solution-3) at 0.01x concentration or with vehicle (saline solution) as the negative control for the treatment. After 24 hours, the cells were lysed with TRIzol RNA Isolation Reagent (1 ml, #15596018; Ambion, Thermo Fisher Scientific) and frozen at -80°C. HEK-293T cells were grown in a humidified 37°C incubator with 5% CO2. The cells were cultured in feeder-free conditions using Dulbecco’s modified Eagle’s medium (41966-029; Gibco) with 10% fetal bovine serum (10270-106; Gibco), 2 mM L-glutamine (25030-024; Gibco), and 1% penicil-lin/streptomycin (P0781; Sigma-Aldrich), with medium changed daily. Cells were dissociated with Trypsin-EDTA solution (T4049, Sigma-Aldrich) when the culture reached ~80% confluency. HEK-293T cells were plated (1000000 cells) and treated with a nutraceutical formula containing propolis, Verbascum, thymus, long chain length polyphosphates, potassium sorbate and sodium benzoate (solution-3) at 0.01x concentration or with vehicle (saline solution) as the negative control for the treatment. After 24 hours, the cells were lysed, and their RNA were extracted. Total RNA, isolated from HEK-293T cells by using TRIzol RNA Isolation Reagent (#15596018; Ambion, Thermo Fisher Scientific) was quantified in a NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermo Scientific™), checked for purity and integrity. Libraries were prepared using the TruSeq® Stranded mRNA Library Kit according to the protocols recommended by the manufacturer. Library kit: TruSeq® Stranded mRNA
description
Unknown
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
kidney
organism part
Nutraceutical mixture-treated (Solution-3); Case
response to treatment
Homo sapiens
scientific_name
HEK293T_Solution3_2
title
Treatment
treatment