2024-06-19
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114247719
External Id
Institute of Oncology research (IOR)
INSDC center name
2023-08-09T19:22:51Z
INSDC last update
public
INSDC status
shCtrl
RNA interference
ERS16234782
SRA accession
E-MTAB-13239:RWPE_KSF_shCtrl_3
Submitter Id
ArrayExpress
broker name
RWPE-1
cell_line
epithelial cell of prostate
cell_type
not collected
collection date
human
common name
Protocols: Experiment in KSF medium. Cells were plated in T-25 flasks at 70% confluence. After 72 h, cells were collected and lysed with 500 µl TRIzol Reagent (15596026, Thermo Fisher Scientific). Experiment in EGM2-medium. Cells were plated in T-25 flasks at 70% confluence with EGM2 medium. After 72 h, EGM2 growth medium was replenished with fresh medium and after 96h cells were collected and lysed with 500 µl TRIzol Reagent (15596026, Thermo Fisher Scientific). RNA was extracted using Direct-zol RNA MiniPrep kit (R2052, Zymo Research) following manufacture instructions. NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was employed with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences.
description
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
prostate gland
organism part
Homo sapiens
scientific_name
Ksf
stimulus
RWPE_KSF_shCtrl_3
title