2024-06-20
ENA first public
ERC000011
ENA-CHECKLIST
Istituto di Ricerche Farmacologiche \"Mario Negri\" IRCCS
INSDC center name
public
INSDC status
ERS18601977
SRA accession
E-MTAB-13942:iPSC_MNCdLS1_H3acetylK27
Submitter Id
ArrayExpress
broker name
induced pluripotent stem cell
cell_type
not collected
collection date
human
common name
Protocols: Briefly, 2-3x107 cells were harvested, fixed with 1 % formaldehyde and quenched with 125 mM glycine. Fixed cells were lysed and the nuclei were extracted. The size of the DNA fragments were adjusted to 300-900 bp long by sonication in the BioRupter sonicator (Diagenor). Immunoprecipitation was performed using the whole genome extracts derived from the cells equivalent to 5x106 cells and 10 g of antibody.  The antibodies used were; H3K4me3 (39159, Active Motif), acetylK27 (ab4729, Abcam), NIPBL (A-301-779A/A-301-778A, Bethyl Laboratories), CTCF (07-729 Merck/348S Cell Signaling), RAD21 (ab952, Abcam/4321S, Cell Signaling).  Small DNA fragments and antibody-protein complexes were purified using protein-G conjugated DynaBeads (Invitrogen). The DNA fragments were reverse-crosslinked and purified by QIAquick PCR purification kit (Qiagen).
description
Cornelia de Lange syndrome
disease
mutation
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
female
sex
iPSC_MNCdLS1_H3acetylK27
title