2024-06-24
ENA first public
ERC000011
ENA-CHECKLIST
RESTORE
INSDC center name
public
INSDC status
ERS20128815
SRA accession
E-MTAB-14120:SC_AT sorted Mph2
Submitter Id
8
week
age
ArrayExpress
broker name
macrophage
cell_type
not collected
collection date
house mouse
common name
Protocols: scAT macrophages were sorted based on CD45+, F4/80+ and CD11b+. AT-macrophages derived from 3D cultures and BM- cultured macrophages were harvested after 13 days in culture.    RNA were extracted from sorted sc-AT macrophages, AT and BM cultured macrophages.  and purified using a Promega extraction kit (Relia PreP ™ RNA Cell Miniprep).  cDNA libraries were generated according to Illumina Stranded mRNA Prep kit (Illumina) from 100ng tRNA. cDNA was amplified for 13 cycles and barcoded with IDT® for Illumina® RNA UD Indexes Set A, Ligation
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
in vivo scAT
growth condition
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
subcutaneous adipose tissue
organism part
Mus musculus
scientific_name
male
sex
C57Bl6/J
strain
SC_AT sorted Mph2
title