2024-06-24
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113956995
External Id
Tettamanti Center, Fondazione IRCCS San Gerardo dei Tintori
INSDC center name
2023-06-29T08:15:13Z
INSDC last update
public
INSDC status
ERS15950983
SRA accession
E-MTAB-13129:Sample 8
Submitter Id
30 months
month
age
ArrayExpress
broker name
chondrocyte
cell_type
not collected
collection date
human
common name
Protocols: To induce cartilaginous differentiation of MPS IH and HD BMSCs the micro-mass culture system was used as previously described (Serafini et al 2014). Pellets were snap frozen Pellets were thawed and homogenized by Mikro-Dismembrator U (Gottingen). Total RNA was extracted using TRIzol reagent (Invitrogen), according to manufacturer&#39;s protocol. Whole transcriptome RNA-seq analyses have been performed by Next Generation Sequencing (NGS), using the Universal RNAseq kit (Nugen, Tecan).The Universal RNA-Seq is an end-to-end solution for strand-specific RNA-Seq library construction used 250 ng nanogram amounts of total RNA obtained from gut sections. The workflow consists of double-stranded cDNA generation using a mixture of random priming, optional fragmentation of double-stranded cDNA, end repair, adaptor ligation, strand selection, targeted transcript depletion with AnyDeplete for globin genes, and PCR amplification to produce the final library. The yields of final libraries were assessed by Qubit 4.0 fluorimeter and their sizing by Agilent Bioanalyzer.
description
postnatal
developmental stage
Hurler syndrome
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
mesenchymal stem cell of the bone marrow
organism part
Homo sapiens
scientific_name
female
sex
Sample 8
title