2024-06-27
ENA first public
ERC000011
ENA-CHECKLIST
Roche Institute for Human Biology (IHB) F Hoffmann-La Roche Early Research and Development
INSDC center name
public
INSDC status
ERS20226746
SRA accession
E-MTAB-14171:P3 IIO
Submitter Id
ArrayExpress
broker name
Tissue resident immune cells + epithelial organoid
cell_type
not collected
collection date
human
common name
Protocols: All the samples were dissociated as follows: matrigel domes containing organoids in coculture with immune cells or immune cells alone were dislodged and mechanically broken up and transferred to 1% BSA-coated tubes. The dome fragments were centrifuged at 400g, 4min, RT. The supernatant was removed and the enzymes of the neural tissue dissociation kit (Miltenyi Biotec) were mixed in HBSS-1%BSA buffer. The matrigel containing the cells were dissociated to single cells for a total of 30 min with thorough pipetting every 7 minutes. Next, cells were filtered through a 40 µM filter and single cells were centrifuged at 450g, 4min, RT. Dead cells were magnetically removed and the viable cell pellet was subsequently resuspended in DPBS 1% BSA. Nuclei acid extraction protocol was performed according to the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index), CG000315, Rev E.  The kit used was: Chromium Next GEM Single Cell 3’ Kit v3.1, 16 rxns PN-1000268. Library Construction protocol was performed according to the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index), CG000315, Rev E.  The kit used was: Library Construction Kit 16 rxns PN-1000190.
description
not collected
geographic location (country and/or sea)
organoid
growth condition
TRM
immune cells
P3
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
small intestine
organism part
Homo sapiens
scientific_name
P3 IIO
title