2024-06-29
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112953988
External Id
INSERM U1242 OSS (Oncogenesis, Stress and Signaling) - Universite de Rennes1
INSDC center name
2023-04-21T15:26:56Z
INSDC last update
public
INSDC status
ERS14949559
SRA accession
E-MTAB-12879:EV
Submitter Id
56
year
age
ArrayExpress
broker name
HuCCT1
cell_line
human
common name
Protocols: 100,000 HuCCT1 pcDNA3.1 (empty vector) and pcLINC00313 over-expressing cells were used per ATAC reaction. Cells were harvested and frozen in culture media containing FBS and 5% DMSO. Cryopreserved cells were sent to Active Motif to perform the ATAC-seq assay. cells were then thawed in a 37°C water bath, pelleted, washed with cold PBS, and tagmented as previously described (Buenrostro et al. 2013), with some modifications based on (Corces et al. 2017). Briefly, cell pellets were resuspended in lysis buffer, pelleted, and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen), amplified with 10 cycles of PCR, and purified using Agencourt AMPure SPRI beads (Beckman Coulter). Resulting material was quantified using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems)
description
adult
developmental stage
intrahepatic cholangiocarcinoma
disease
wild type genotype
genotype
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
liver
organism part
wild type
phenotype
Homo sapiens
scientific_name
male
sex
EV
title