2024-06-30
ENA first public
ERC000011
ENA-CHECKLIST
USC
INSDC center name
public
INSDC status
ERS20220437
SRA accession
E-MTAB-14167:AD 10 Cbl
Submitter Id
88
year
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Tissues were received from NIH brain banks from postmortem individuals. Diagnoses were assigned by a pathologist.  20mg of brain tissue was homogenized in TRIzol reagent using a BeadBug Benchtop Homogenizer. Brain tissue was suspended in 1 mL of TRIzol and homogenized for 6 rounds of 10 s homogenization and 60 s holds between each round. 300 µL of chloroform was added to the sample and aqueous separation of RNA was performed using centrifugation in a heavy gel phase-lock tube (VWR, 10847-802). The aqueous phase was applied to a standard column-based RNA purification kit (Quantabio, Extracta Plus, Cat# 95214-050) following the manufacturer’s protocol.  Preparation of libraries for sequencing was handled by Novogene.
description
adult
developmental stage
Alzheimer's disease
disease
ApoE3,3
genotype
not collected
geographic location (country and/or sea)
971
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
cerebellum
organism part
Homo sapiens
scientific_name
male
sex
AD 10 Cbl
title