2024-06-30
ENA first public
ERC000011
ENA-CHECKLIST
University of Veterinary Medicine Vienna Institute of Pharmacology and Toxicology
INSDC center name
public
INSDC status
ERS18269459
SRA accession
E-MTAB-13798:514-2_cdk6_HA-deltaC_ChIP
Submitter Id
ArrayExpress
broker name
514-2
cell_line
hematopoietic cell
cell_type
not collected
collection date
house mouse
common name
Protocols: Cells expressing CDK6_WT, CDK6_deltaC, as well as CDK6_KO cells were harvested from cell line culture. Cells were crosslinked with DSG (20 minutes, RT) and 1% formaldehyde (10 minutes, RT) and termination of the fixation procedure was performed using glycine. For immunoprecipitation (IP), 70 µl Dynabeads Protein G magnetic beads (Invitrogen) were used. The antibody against the HA tag was ab9110 (Abcam).  Input DNA or DNA enriched through IP was subjected to sequencing library preparation using the NEBNext Ultra II chemistry (New England Biolabs)
description
adult
developmental stage
leukemia
disease
CDK6_deltaC
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
bone marrow cell
organism part
Mus musculus
scientific_name
514-2_cdk6_HA-deltaC_ChIP
title