2024-06-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA111457718
External Id
College of Medicine, Health and Life Science, Brunel University London, Center of Genomic Engineering and Maintenance (CenGem)
INSDC center name
2022-10-12T13:07:44Z
INSDC last update
public
INSDC status
ERS13551754
SRA accession
E-MTAB-12279:Mitosis_Thymidine_Auxin_1
Submitter Id
ArrayExpress
broker name
Mitosis
cell cycle phase
HCT116
cell_line
human
common name
Protocols: Ki-67 was degraded in cells arrested in prometaphase and released in thymidine for 18h. Then the sample was collected Total RNA extraction was performed with the Monarch Total RNA Miniprep Kit (New England Biolabs, Hitchin, UK) according to the manufacturer’s protocol. Libraries generated with TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) according to the manufacturer’s protocol.
description
adult
developmental stage
colorectal carcinoma
disease
AID degron-Ki-67
genotype
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
mixed large intestine and colon
organism part
Ki-67 degraded
phenotype
Homo sapiens
scientific_name
Mitosis_Thymidine_Auxin_1
title