2024-07-01
ENA first public
ERC000011
ENA-CHECKLIST
AZ
INSDC center name
public
INSDC status
ERS18331571
SRA accession
E-MTAB-13831:HCC827_YAP1_DMSO_48h_R3
Submitter Id
ArrayExpress
broker name
HCC827
cell_line
immortal cell line cell
cell_type
not collected
collection date
human
common name
Protocols: ~2 million cells per sample were lysed in 350uL of RLT and stored at -80 until subsequent extraction. RNA was extracted using the RNeasy 96 QIAcube HT Kit (Qiagen) with a DNAse digest as per manufactures recommendations. Libraries were prepared using mRNA Stranded library preparation kit (Illumina) and subsequently quantified by Qubit and KAPA library quantification kit, ROX low (Roche). Library sizes were also determined by bioanalyzer.
description
adult
developmental stage
non-small cell lung adenocarcinoma
disease
YAP1
genetic modification
EGFR mutant
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
lung
organism part
3
replicate
Homo sapiens
scientific_name
HCC827_YAP1_DMSO_48h_R3
title