2024-07-01
ENA first public
ERC000011
ENA-CHECKLIST
Duke University
INSDC center name
public
INSDC status
ERS18400256
SRA accession
E-MTAB-13889:1-1
Submitter Id
5
day
age
ArrayExpress
broker name
not collected
collection date
Japanese rice
common name
Kitaake
cultivar
Protocols: Sections (root tip to end of lateral root cap: Meristem; end of lateral root cap to the start of root hair elongation: Elongation; 1mm each beyond the start of root hair elongation: Maturation1 and Maturation2) were harvested into 10 μL RNA-later (Ambion) in the lid of a 1.5 ml tube. Samples were frozen in liquid nitrogen and stored at −80C X.kitaake was used as wild type. Rice seeds were dehulled, sterilized with 50% bleach for 10 min, and rinsed four to five times with sterile water. All plant growth was in Yoshida’s nutrient solution solidified with gellan gum (Gelzan, Caisson Inc).Growth boxes were kept at 4°C in the dark for 2-3 days until the seeds germinate, then transferred to a percival growth chamber set to 28°C, and consistant light. Rice seedlings were grown in the percival for 1-3 days before harvesting. Tissue samples were processed by grinding with a blue homogenization pestle. RNA was isolated using the Zymo MagBead RNA Isolation kit according to the manufacturer’s protocol (Zymo). RNA was used as input into the Lexogen QuantSeq 3′ FWD RNA-Seq library preparation procedure according to the manufacturer’s protocol, using the Unique Molecular Identifier (UMI) PCR add-on kit.
description
Meristem
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Oryza sativa Japonica Group
organism
Oryza sativa Japonica Group
organism
root
organism part
Oryza sativa Japonica Group
scientific_name
1-1
title