2024-07-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115057825
External Id
Bioinformatic Solutions LLC
INSDC center name
2023-12-22T14:19:24Z
INSDC last update
public
INSDC status
ERS17598321
SRA accession
E-MTAB-13674:CGD01 V4
Submitter Id
28.5 YO
year
age
ArrayExpress
broker name
neutrophils
cell_type
not collected
collection date
human
common name
IFNG
compound
Protocols: IFN-γ administration was initiated at the dose of 50 mcg/m2 given subcutaneously on the standard schedule of Monday, Wednesday, and Friday in the evening before bedtime. Then, 10-12 hours after the 1st dose, and the 4th dose of IFN-γ additional blood samples were obtained. Neutrophils were isolated from heparinized peripheral blood by Dextran sedimentation, Ficoll-Hypaque density centrifugation, and hypotonic lysis of red blood cells 36,37. Neutrophils were quantitated manually in a hemocytometer in the presence of 3% paraformaldehyde. RNA from neutrophils was isolated by standard technique. RNA was used to prepare libraries using the Universal Plus mRNA-seq kit from Tecan Genomics (Redwood City, CA).
description
adult
developmental stage
chronic granulomatous disease
disease
50
microgram per kilogram per day
dose
Blood draw 10-12 hours after the 4th dose
hour
dosing time
not collected
geographic location (country and/or sea)
CGD01
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
male
sex
CGD01 V4
title