2024-07-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113945349
External Id
Shanghai Institute of Nutrition and Diseases, Chinese Academy of Sciences
INSDC center name
2023-06-27T11:02:12Z
INSDC last update
public
INSDC status
ERS15939599
SRA accession
E-MTAB-13114:Kdm4b IP
Submitter Id
22
hour
age
ArrayExpress
broker name
not collected
collection date
zebrafish
common name
Protocols: WT and kdm4b mutant embryos at 22 hpf were fixed in 1% formaldehyde and then lysed in SDS buffer on ice for 30 minutes. Chromatin was sonicated by Fisher Scientific’s Sonic Dismembrator with 30% amplitude, pulse for 10 s on and 30 s off for a total sonication ‘on’time of 3 min 30 s to generate 100~500 bp fragment DNA. Sonicated chromatin was precipitated by antibodies specific for H3K9me3 (ab8898, Abcam), H3K36me3 (ab9050, Abcam), FOXO3 (ab12162, Abcam), or Normal Rabbit IgG (Negative control, 2729, CST). 10 ng of DNA samples were prepared for Illumina sequencing as the standard steps. Illumina's genomic adapters were ligated to the DNA fragments. Enriched ligated fragments of ~200 - 500 bp was purified and quantified. The libraries were denatured, captured on Illumina flow cell and amplified in situ.
description
embryo
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
mixed pool of 200 embryos
individual
not applicable
isolate
Danio rerio
organism
Danio rerio
organism
whole organism
organism part
Danio rerio
scientific_name
mixed
sex
Tubingen
strain
Kdm4b IP
title