2024-07-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115121262
External Id
Institute of Biochemistry Justus-Liebig University Giessen
INSDC center name
2024-01-20T13:28:22Z
INSDC last update
public
INSDC status
ERS17788887
SRA accession
E-MTAB-13714:WT1_input_t9
Submitter Id
ArrayExpress
broker name
not collected
collection date
fission yeast
common name
Protocols: Cells were grown in EMMG, then shifted to YES for 9h. S. cerevisiae spike-in cells were added at at 1:5 OD600 ratio immediately before crosslinking with 1% formaldehyde for 20 min at room temperature. ChIP experiments were performed as described in Wittmann et al., Nat Commun 8, 14861 (2017). RNAPII was immunoprecipitated with antibody against Rpb1 (8WG16, Millipore) coupled to 20 µl of protein-G dynabeads (Life Technologies). Libraries were prepared with the NEBNext Fast DNA Library Prep Set for Ion Torrent Kit (NEB).
description
h+ leu1-32 ura4D18 ade6-M216 dbp2::dbp2-3myc:kanMX
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Schizosaccharomyces pombe
organism
Schizosaccharomyces pombe
organism
whole organism
organism part
Schizosaccharomyces pombe
scientific_name
YP805
strain
WT1_input_t9
title