2024-07-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113945416
External Id
Shanghai Institute of Nutrition and Diseases, Chinese Academy of Sciences
INSDC center name
2023-06-27T11:19:32Z
INSDC last update
public
INSDC status
ERS15939666
SRA accession
E-MTAB-13116:WT3
Submitter Id
48
hour
age
ArrayExpress
broker name
erythrocyte
cell_type
not collected
collection date
zebrafish
common name
Protocols: The 48 hpf gata1+ erythrocytes were sorted (FACSAria, BD Bioscience, CA, USA) Two independent groups of RNA from wt and kdm4b-/- erythrocytes were extracted using TRIzol® Reagent (Life Technologies) (each group contains three biological repeats). After adenylation of 3’ ends of double strand cDNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. The library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase. At last, PCR products were purified and the library quality was assessed on the Agilent Bioanalyzer 2100 system.
description
embryo stage
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
gata1 positive
immunophenotype
mixed pool of 200 individuals
individual
not applicable
isolate
Danio rerio
organism
Danio rerio
organism
embryo
organism part
Danio rerio
scientific_name
mixed
sex
Tubingen
strain
WT3
title