2024-07-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115057835
External Id
Bioinformatic Solutions LLC
INSDC center name
2023-12-22T14:19:24Z
INSDC last update
public
INSDC status
ERS17598331
SRA accession
E-MTAB-13674:CGD05 V2
Submitter Id
5.3 YO
year
age
ArrayExpress
broker name
neutrophils
cell_type
not collected
collection date
human
common name
IFNG
compound
Protocols: IFN-γ administration was initiated at the dose of 50 mcg/m2 given subcutaneously on the standard schedule of Monday, Wednesday, and Friday in the evening before bedtime. Then, 10-12 hours after the 1st dose, and the 4th dose of IFN-γ additional blood samples were obtained. Neutrophils were isolated from heparinized peripheral blood by Dextran sedimentation, Ficoll-Hypaque density centrifugation, and hypotonic lysis of red blood cells 36,37. Neutrophils were quantitated manually in a hemocytometer in the presence of 3% paraformaldehyde. RNA from neutrophils was isolated by standard technique. RNA was used to prepare libraries using the Universal Plus mRNA-seq kit from Tecan Genomics (Redwood City, CA).
description
juvenile
developmental stage
chronic granulomatous disease
disease
50
microgram per kilogram per day
dose
Baseline blood draw
hour
dosing time
not collected
geographic location (country and/or sea)
CGD05
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
male
sex
CGD05 V2
title