2024-07-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113937328
External Id
Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium
INSDC center name
2023-06-23T14:42:16Z
INSDC last update
public
INSDC status
ERS15931489
SRA accession
E-MTAB-13112:NAA24h_2
Submitter Id
3
day
age
ArrayExpress
broker name
not collected
collection date
thale cress
common name
Col-0
cultivar
Protocols: root sections between 1000 µm and 2000 µm from the root tip were microdissected Col-0 seeds were germinated on medium containing 10 μM NPA and transferred 3 d after germination under continuous light to 10 μM NAA, according to the points of the time course. total RNA was extracted using Trizol (Invitrogen) and the RNeasy Plant mini kit (Qiagen) Using the Illumina TruSeq® Stranded mRNA Sample Prep Kit poly-A containing mRNA molecules were purified from the total RNA input using poly-T oligo-attached magnetic beads. In a reverse transcription reaction using random primers, RNA was converted into first strand cDNA and subsequently converted into double-stranded cDNA in a second strand cDNA synthesis reaction using DNA PolymeraseI and RNAse H. The cDNA fragments were extended with a single 'A' base to the 3' ends of the blunt-ended cDNA fragments after which indexing adapters were ligated introducing single distinct barcodes for each sample. Finally, enrichment PCR was carried out to enrich those DNA fragments that have adapter molecules on both ends and to amplify the amount of DNA in the library.
description
seedling development stage
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Arabidopsis thaliana
organism
Arabidopsis thaliana
organism
root
organism part
root 1-2 mm from tip
sampling site
Arabidopsis thaliana
scientific_name
NAA24h_2
title