2024-07-02
ENA first public
ERC000011
ENA-CHECKLIST
Kyong Hee University
INSDC center name
public
INSDC status
ERS18631891
SRA accession
E-MTAB-13965:WT-3
Submitter Id
ArrayExpress
broker name
not collected
collection date
Japanese rice
common name
Dongjin
cultivar
Protocols: The samples were collected from paddy field at anthesis stage. We froze the anthers immediately using liquid nitrogen. Plants were transplanted to paddy field after two weeks in incubator at BOD incubator at end of May. The plants were then grown for 3 months in the field. Mature anthers were frozen in LN2 and finely ground using mortar. Grounded samples were homogenized using TRI reagents (RNAiso, Takara) and BCP (1-bromo-3-chloropropane). DNA, RNA, and protein layers were separated by centrifugation. Then, total RNA was extracted using the RNeasy Mini Kit (QIAGEN). The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, \\"tagmentation\\" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Oryza sativa Japonica Group
organism
Oryza sativa Japonica Group
organism
anther
organism part
Oryza sativa Japonica Group
scientific_name
WT-3
title