2024-07-08
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA110292087
External Id
Advanced Centre for Treatment Research and Education in Cancer (ACTREC)
INSDC center name
2022-07-08T14:44:18Z
INSDC last update
public
INSDC status
ERS12389695
SRA accession
E-MTAB-11925:AD1964_UNTREATED
Submitter Id
ArrayExpress
broker name
MDA-MB-231
cell_line
human
common name
Protocols: Each sample was collected by scrapping the cells from a 10cm cell culture dish. Pellet was stored at -20 degrees. T47D and MDA-MB-231 cells were cultured in RPMI and DMEM medium respectively. The media was supplemented with 10% FBS, 2.5 mg/ml Amphotericin-B (Abbott) and 1.25 μl/ml Gentamycin (Abbott). The cells were cultured at 37 °C in a 5% CO2 incubator. Cells were serum starved followed by treatment with 10nM of progesterone for 6 hours. Equal volume of ethanol was used as vehicle control RNA was extracted using the TRIzol solubilization and extraction protocol, as recommended by the manufacturer. Transcriptome sequencing libraries were constructed by using the TruSeq RNA library protocol (Illumina). Briefly, ribosomal RNA was depleted and library preparation was done as per the manufacturer's instructions (TruSeq RNA Sample Preparation Kit, Illumina).
description
adult
developmental stage
breast cancer
disease
wild type genotype
genotype
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
breast
organism part
Homo sapiens
scientific_name
AD1964_UNTREATED
title