2024-07-09
ENA first public
ERC000011
ENA-CHECKLIST
German Cancer Research Center (DKFZ), Heidelberg Technical University Munich, School of Medicine
INSDC center name
public
INSDC status
ERS20394794
SRA accession
E-MTAB-14206:113
Submitter Id
ArrayExpress
broker name
CD45 cell
cell_type
not collected
collection date
house mouse
common name
Protocols: The entire colon was removed from either Hsp60Δ/ΔIEC or Hsp60fl/fl mice weighed and stored in Dulbecco's Modified Eagle Medium (DMEM). After being washed with PBS and minced on ice, tissue specimens were dissociated and enzymatically digested (Multi Tissue Dissociation Kit using gentleMACS™ Dissociator, Miltenyi Biotec). After centrifugation and resuspension in PBS+2% FCS, the cell suspension was strained through a 70-µm strainer, spun down and resuspended in 2% FCS/PBS including RNase inhibitor (New England Biolabs, catalog no. M0314L,1:100). Debris removal solution (Miltenyi Biotec, catalog no. 130-109-398) was used to remove cell debris from the dissociated tissue. Then the dead cell removal kit (Miltenyi, catalog no. 130-090-101) was used to enrich live cells. CD45+ cells were sorted out using the CD45 Microbeads kit (Miltenyi Biotec, catalog no. 130-052-301).  https://cdn.10xgenomics.com/image/upload/v1660261285/support-documents/CG000204_ChromiumNextGEMSingleCell3_v3.1_Rev_D.pdf https://cdn.10xgenomics.com/image/upload/v1660261285/support-documents/CG000204_ChromiumNextGEMSingleCell3_v3.1_Rev_D.pdf
description
Hsp60Δ/ΔIEC (inducible deletion of the mitochondrial chaperone Hsp60 in intestinal epithelial cells )
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
colon
organism part
Mus musculus
scientific_name
113
title