2024-07-12
ENA first public
ERC000011
ENA-CHECKLIST
Leeds institute of cardiovascular and metabolic medicine, University of Leeds, Leeds, UK
INSDC center name
public
INSDC status
ERS20120747
SRA accession
E-MTAB-14110:Sample 14
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Skeletal muscle biopsies from the pectoralis major were collected from each patient, frozen in liquid nitrogen, and stored at -80°C. Unbiased RNA sequencing was performed from these biopsies.  RNA extraction was performed using the trizol-chloroform-isopropanol method following Trizol reagent solution user guide (ThermoFisher Scientific). RNA purification using the RNA clean and concentrate-5 kit (Zymo) was conducted following the kit protocol resulting in high quality RNA in 20 µl of RNase-free water, confirmed using a nanodrop.  cDNA libraries are sequenced using Illumina NovaSeq platforms, which utilise a paired-end 150 bp sequencing strategy.
description
normal
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
skeletal muscle tissue
organism part
Homo sapiens
scientific_name
female
sex
Sample 14
title