2024-07-13
ENA first public
ERC000011
ENA-CHECKLIST
Unistra
INSDC center name
public
INSDC status
ERS20302621
SRA accession
E-MTAB-14200:M3
Submitter Id
13
week
age
ArrayExpress
broker name
mesenchymal cell
cell_type
not collected
collection date
house mouse
common name
Protocols: Tumor tissue was collected 11 weeks after grafting. Tumor samples were fresh frozen. Total RNA was extracted using RNeasy® Plus Micro Kit (Qiagen) according to the manufacturer’s instructions. Libraries were prepared from 10 ng RNA using SMARTer Stranded Total RNA-Seq Kit, Pico Input Mammalian (Takara) following the manufacturer’s instructions. Briefly, random primers were used for first-strand synthesis, and ribosomal cDNA was cleaved by ZapR V.2 in the presence of mammalian R-probes V.2.
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
M3
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Tumor
organism part
Mus musculus
scientific_name
female
sex
C57Bl6
strain
M3
title