2024-07-16
ENA first public
ERC000011
ENA-CHECKLIST
University of Birmingham
INSDC center name
public
INSDC status
ERS18229334
SRA accession
E-MTAB-13790:Untagged H-NS control with H-NS-39 sample A
Submitter Id
ArrayExpress
broker name
AB5075 pVRL1Zhns-39
cell_line
not collected
collection date
Protocols: A. baumannii AB5075 is a clinical strain isolated from an infected tibia.Jacobs, A. C. et al. AB5075, a Highly Virulent Isolate of Acinetobacter baumannii, as a Model Strain for the Evaluation of Pathogenesis and Antimicrobial Treatments. (2014) doi:10.1128/mBio.01076-14. Cells were grown in liquid LB medium at 37 degrees with shaking. Immunoprecipitations were done as described by Haycocks et al. (PLoS Genet. 2019. 15, e1008362) using A. baumannii strain AB5075. After growing cells to midlog phase, crosslinking, and sonication, protein DNA complexes were immunoprecipitated using anti-FLAG.   Libraries were prepared using immunoprecipited protein-DNA complexes immobilised with Protein A-sepharose. DNA fragments were then blunt ended, A-tailed and barcoded. This was done using an NEB quick blunting and ligation kit (NEBNext Ultra II DNA Library Prep Kit for Illumina) and NEBNext Oligos for Illumina. Following elution of complexes from the Protein-A sepharose, crosslinks were reversed and barcoded libraries were amplified by PCR. The number of PCR cycles was determined empirically for each library. After amplification, library concentration was quantified using a Tapestation and real time PCR.
description
wild type  + pVRL1Zhns-39
genotype
not collected
geographic location (country and/or sea)
mid logarithmic growth
growth conditions
not applicable
isolate
Acinetobacter baumannii
organism
Acinetobacter baumannii
organism
whole organism
organism part
A
replicate
Acinetobacter baumannii
scientific_name
AB5075
strain
Untagged H-NS control with H-NS-39 sample A
title