2024-07-17
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113597068
External Id
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SA, UK University of Cambridge, Department of Haematology, Cambridge, CB2 0AW, UK Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute, Cambridge, CB2 0AW, UK
INSDC center name
2023-06-13T16:09:52Z
INSDC last update
public
INSDC status
ERS15591161
SRA accession
E-MTAB-13062:TS21_4_A
Submitter Id
13
week
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Tissues were frozen in dry-ice cooled isopentane and stored in air-tight tissue cryovials at -80°C. Prior to undertaking any spatial transcriptomics protocol, the tissues were embedded in an optimal cutting temperature compound (OCT) and tested for RNA quality with RNA integrity number (RIN). Tissues with RIN values >7 were cryosectioned in a pre-cooled cryostat at 10 μm thickness. Two consecutive sections were cryosectioned at 10 μm thickness in a pre-cooled cryostat and transferred to the four 6.5mm x 6.5mm capture areas of the gene expression slide. Slides were fixed in methanol for 30 minutes prior to staining with H&E and then imaged using the Nanozoomer slide scanner. The tissues underwent permeabilization for 6 minutes. Reverse transcription and second strand synthesis was performed on the slide with cDNA quantification using qRT-PCR using KAPA SYBR FAST-qPCR kit (KAPA Biosystems) and analysed on the QuantStudio (ThermoFisher). Following library construction, these were quantified and pooled at 2.25 nM concentration. Reverse transcription and second strand synthesis was performed on the slide with cDNA quantification using qRT-PCR using KAPA SYBR FAST-qPCR kit (KAPA Biosystems) and analysed on the QuantStudio (ThermoFisher). Following library construction, these were quantified and pooled at 2.25 nM concentration.
description
embryo stage
developmental stage
Down's syndrome
disease
not collected
geographic location (country and/or sea)
TS21_4
individual
not applicable
isolate
trisomy 21
karyotype
Homo sapiens
organism
Homo sapiens
organism
liver
organism part
DS 15582
original source name
Homo sapiens
scientific_name
TS21_4_A
title