2024-07-19
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114510430
External Id
The University of Manchester, UK
INSDC center name
2023-10-18T13:18:19Z
INSDC last update
public
INSDC status
ERS16492077
SRA accession
E-MTAB-13454:M4
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Tissue samples from hair transplant surgeries were collected with written informed consent and ethics approval granted from UK North-West Haydock Research Ethics Committee (19/NW/0082). Isolated full-length anagen VI hair follicles were cultured 24hr as previously described (PMID: 26284830). Media was replaced every 48hrs with the following additions: Cobimetinib 1 μM (Selleckchem); or vehicle control DMSO (0.5%). Total RNA was extracted from 6-8 full-length anagen VI hair follicles using the RNAeasy mini kit (Qiagen, Manchester, UK) according to manufacturer’s protocol. Total RNA was submitted to the University of Manchester Genomic Technologies Core Facility (GTCF). Illumina TruSeq Stranded mRNA kit, according to the manufacturer’s protocol.
description
adult
developmental stage
not collected
geographic location (country and/or sea)
4
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
hair follicle
organism part
Homo sapiens
scientific_name
male
sex
M4
title