2024-07-19
ENA first public
ERC000011
ENA-CHECKLIST
EMBL
INSDC center name
public
INSDC status
ERS20568704
SRA accession
N2 #1
Submitter Id
2023-02-22
collection date
Single embryos were collected from NGM plates 24h post backcrossing L4-staged N2/CB4856  heterozygous hermaphrodites with CB4856 males. Samples were frozen in 5 uL of 1XDreamTaq PCR buffer and 1 mg/mL of Proteinase K and stored at -80°C. Genomic DNA was extracted after incubation at 65°C for 1h followed by Proteinase K inactivation at 95°C for 15 min. Genomic DNA was amplified using the PicoPLEX Single Cell WGA Kit (Takara) followed by DNA bead clean up using 1.8X SRPI beads. Tn5-based DNA library was prepared by the EMBL Genomics Core Facility and 300 bp paired-end sequencing was performed on a NextSeq2000 instrument.
description
embryo
dev_stage
Germany
geographic location (country and/or sea)
Caenorhabditis elegans
organism
Caenorhabditis elegans
scientific_name
N2oogenesis9
title