2024-07-22
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112849608
External Id
Oxford University IDRM, Department of Paediatrics
INSDC center name
2023-03-29T13:58:28Z
INSDC last update
public
INSDC status
ERS14856229
SRA accession
E-MTAB-12713:dSpin_2
Submitter Id
ArrayExpress
broker name
house mouse
common name
Protocols: FACS purified P14 spermatogonial stem cells DNA from spermatogonia was isolated by proteinase K digest (10 mM Tris-HCl pH 8, 5 mM EDTA , 1% SDS, 0.3 M Na-acetate, 0.2 mg/ml proteinase K) overnight, followed by 2x phenol/chloroform/isoamylalcohol (25:24:1, Sigma-Aldrich) extraction and 1x chloroform extraction. The DNA was precipitated at -20˚C after addition of 1/10 volume 3M Na-acetate, 10µg linear acrylamide (Invitrogen) and 1 volume of isopropanol, washed 2x and solubilized in 5mM Tris pH8. Methyl-seq libraries were prepared using the NEBnext Enzymatic Methyl-seq kit (NEB) according to the manufacturer’s instructions
description
juvenile stage
developmental stage
dSpin knockout
genotype
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
testis
organism part
Mus musculus
scientific_name
male
sex
C57BL/6N
strain
dSpin_2
title