1000-01-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114461055
External Id
University of Sheffield
INSDC center name
2023-10-02T09:25:06Z
INSDC last update
public
INSDC status
ERS16447699
SRA accession
E-MTAB-13383:H7 1Q R3
Submitter Id
ArrayExpress
broker name
H7-hESC
cell_line
pluripotent stem cell
cell_type
not collected
collection date
human
common name
Protocols: Three biological replicates of wild-type and v1q sublines of the H7 and H9hPSC lines from E8/VTN cultures were used for RNA extraction and RNAseq analysis. Prior to RNA isolation, cells were lysed using Buffer RLT (Cat. # 79216; Qiagen) and stored at -80C. Human pluripotent stem cell culture For E8/VTN culture conditions, hPSC were grown on vitronectin (VTN-N) (Cat. # A14700, Life Technologies)-coated flasks (5 µg/ml in Dulbecco’s phosphate buffered saline (PBS) without Calcium and Magnesium) in modified E8 medium11 prepared in house, consisting of DMEM/F12 (Cat. # D6421; Sigma-Aldrich) supplemented with 14 µg/l sodium selenium (Cat. # S5261; Sigma-Aldrich), 19.4 mg/l insulin (Cat. # A11382IJ; Thermo Fisher Scientific), 1383 mg/l NaHCO3 (Cat. # S5761; Sigma-Aldrich), 10.7 mg/l transferrin (Cat. # T0665; Sigma-Aldrich), 10 ml/l Glutamax (Cat. # 35050038; Thermo Fisher Scientific), 40µg/l FGF2-3 (Cat. # Qk053; Qkine) and 2 µg/l TGFβ1 (Cat. # 100-21; Peprotech). For time lapse experiments, E8 was prepared using DMEM/F12 without phenol red (Cat. # D6434; Sigma-Aldrich). Cells were fed daily and maintained at 37°C under a humidified atmosphere of 5% CO2 in air. RNA was isolated, libraries constructed and sequenced by GENEWIZ (Azenta Life Sciences, UK). libraries were prepared for Illumina (New England Biolabs, Ipswich, USA) and the library preparations were sequenced on an Illumina Novaseq platform (Illumina, San Diego, USA) to generate 150 bp paired-end reads.
description
embryo stage
developmental stage
normal
disease
46,XX,dup(1)(q21q42)
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
inner cell mass
organism part
Homo sapiens
scientific_name
H7 1Q R3
title