SAMN00110598
External Id
University of Guelph
INSDC center name
2011-08-08T00:00:00Z
INSDC first public
2022-12-19T21:07:40.897Z
INSDC last update
SRS114513
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS114513
SRA accession
The focus of this project was to develop a method for identifying SNPs in a fungal genome using a pooled sample of DNA from multiple genotypes using 454 pyrosequencing when no reference sequence is available. We had no previous knowledge of the genome of Oc-j. Therefore, eight isolates, including the type culture of Oc-j (ATCC 36642) were selected for analysis. The isolates were chosen for their geographic distribution and phenotypic characteristics. Total nucleic acid quantities in these samples ranged from 1600 ng to 3000 ng for the eight isolates. DNA extracted from the eight isolates was pooled in an attempt to maximize the diversity in the genomes sampled.
description
Ophiognomonia clavigignenti-juglandacearum
http://purl.obolibrary.org/obo/NCBITaxon_218668
organism
Genomic DNA was a pooled sample consisting of eight strains of Ophiognomonia clavigignenti-juglandacearum (ATCC 36624, SCJ2, SCJ3, P005, WB22, GA11, GA51, and BUD23)
orgmod_note
Pooled sample containing eight isolates of Ophiognomonia clavigignenti-juglandacearum
title