LIBEST_020370
External Id
SAMN00166277
External Id
University of British Columbia
INSDC center name
2006-09-12T00:00:00Z
INSDC first public
2023-10-26T21:06:24.351Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS16610499
SRA accession
Mycelia for library construction were collected from solid media inoculated with a suspension containing 5x105 spores and were incubated for 7 days under ambient conditions. Mycelia were generated on media containing 1% Difco MEA mixed with 1.5% (w/v) autoclaved agar. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA libraries were normalized according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791]. The MPB02 library was normalized to the Cot 5 level.
description
Harvested after 7 days growth
dev-stage
Leptographium clavigerum
http://purl.obolibrary.org/obo/NCBITaxon_226899
organism
EcoRI (5' end of cDNA)
re_1
XhoI (3' end of cDNA)
re_2
SLKw1407
strain
Mixed hyphal
tissue-type
MPB02
title
pBluescript II SK (+) XR
vector