SAMN00167254
External Id
LIBEST_024276
External Id
Dipartimento di Ortofloroarboricoltura e Tecnologie Agroalimentari, Sezione Arboricoltura, University of Catania,
INSDC center name
2009-01-30T00:00:00Z
INSDC first public
2023-09-28T21:06:08.147Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS16610504
SRA accession
Comune and Monreal
cultivar
Total RNA was extracted using Plant RNA Reagent (Invitrogen) from unpollinated and self-pollinated pistils of 'Comune' clementine and its self-compatible natural mutation 'Monreal', grown at the experimental station of Catania University. About 100 ug total RNA was used to isolate mRNA by PolyATract mRNA Isolation System III (Promega). cDNA synthesis and cDNA-AFLP analysis were carried according to Bachem et al (1996). Differentially expressed and polymorphic sequences were visually screened and excised from gels, eluted overnight with water, reamplified with the same conditions used for selective amplifications and electrophoresed in 1% agarose gel. All the successfully reamplified TDFs were excised from agarose, purified with QIAquick gel extraction kit (Qiagen), cloned into pgem-T Easy vector (Promega), and sequenced using an ABI310 genetic analyzer (Applied Biosystems). Seqman software (DNAstar, Madison,WI, USA) was used to remove vector sequences and to find possible overlaps among the transcript derived fragments.
description
Citrus x clementina
http://purl.obolibrary.org/obo/NCBITaxon_85681
organism
EcoRI
re_1
MseI
re_2
Unpollinated pistils or self-pollinated pistils
tissue-type
Citrus clementina pistils cDNA-AFLP library
title
pGEM-T easy
vector