SAMN00169196
External Id
LIBEST_026130
External Id
Natural Resources Canada
INSDC center name
2010-03-11T00:00:00Z
INSDC first public
2022-10-14T21:06:42.209Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS20623710
SRA accession
resting and germinating urediniospores, germ tubes, epiphytic mycelia, etc.
cell type
Naturally infected leaves were collected in September. Epiphytic materials corresponding to resting and germinating uredinospores, germ tubes, epiphytic mycelia and uredinia were scraped and characterized by qPCR to ensure there was no cross-contamination (Boyle et al. 2005. Appl. Environ. Microbiol. 71, 1546-1552; RNA was obtained by grinding material with a Mixer Mill MM300; mRNA purification using Dynabeads; cDNA preparation using Stratagene pBluescript II XR cDNA library construction kit.
description
epiphytic materials at different growth stages scraped from infected poplar (Populus balsamifera x P. maximowiczii '733502')
dev-stage
Natural field infection
isolate
E. coli ElectroMAXTM DH10BTM
lab-host
Melampsora laricis-populina
http://purl.obolibrary.org/obo/NCBITaxon_203908
organism
EcoRI (5'-end)
re_1
XhoI (3'-end)
re_2
MLP-E
title
pBluescript II XR
vector