LIBEST_023774
External Id
SAMN00166733
External Id
DOE Joint Genome Institute
INSDC center name
2008-10-07T00:00:00Z
INSDC first public
2022-09-01T21:05:19.086Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS20623678
SRA accession
The library was made from oligo(dT)-primed cDNA and cloned into the pMCL200_cDNA vector. PolyA RNA was primed with oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected >2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested pMCL200_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).
description
24 hour culture
dev-stage
Fusarium vanettenii
http://purl.obolibrary.org/obo/NCBITaxon_2747968
organism
SalI
re_1
NotI
re_2
77-13-4
strain
mycelium and spores
tissue-type
CATI Nectria haematococca mpVI Sporulation (PDB) 77-13-4 Mycelia 24 hour culture
title
pMCL200_cDNA
vector