SAMN00002233
External Id
USDA-ARS
INSDC center name
2009-04-22T00:00:00Z
INSDC first public
2023-07-24T21:06:18.232Z
INSDC last update
SRS002353
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS002353
SRA accession
Honey bees infected with N. ceranae were collected from the USDA-ARS Bee Research Laboratory apiaries, Beltsville, MD. Alimentary tracts of these bees were removed and crushed in sterile water and filtered through a Corning (Lowell, MA) Netwell insert (24 mm diameter, 74 μm mesh size) to remove tissue debris. The filtered suspension was centrifuged at 3,000 x g for 5 minutes and the supernatant discarded. The re-suspended pellet was further purified on a discontinuous Percoll (Sigma-Aldrich, St. Louis, MO) gradient consisting of 5 ml each of 25%, 50%, 75% and 100% Percoll solution. The spore suspension was overlaid onto the gradient and centrifuged at 8,000 x g for 10 minutes at 4o C. The supernatant was discarded and the spore pellet was washed by centrifugation and suspension in distilled sterile water.
description
infected adults
isolation-source
Apis mellifera
http://purl.obolibrary.org/obo/NCBITaxon_7460
nat-host
Vairimorpha ceranae BRL01
http://purl.obolibrary.org/obo/NCBITaxon_578460
organism
BRL01
strain
Generic sample from Nosema ceranae BRL01
title