SAMN00176516
External Id
LIBEST_017660
External Id
Instituto Tecnologico de Celaya, Departamento de Ingenieria Bioquimica
INSDC center name
2005-07-25T00:00:00Z
INSDC first public
2022-05-05T21:08:31.356Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS20623716
SRA accession
2 ul PCR fragments were ligated with 1 ul pCR2.1-TOPO cloning vector according to the manufacturer's instructions (Invitrogen, Carlsbad, CA, USA). 2 ul ligation reaction solutions were transformed into 50 ul E. coli chemically competent cells strain TOP 10. The transformation culture was plated on Petri dishes containing LB/kamamycin/IPTG/X-gal, and the screened white colony for insert fragment. Selected white colonies containing recombinant plasmid were inoculated separately into 5 ml LB/ampicillin/kanamycin solution and it was shaken at 37 oC overnight. Then 500 ul each culture were added into 2 ml cryogenic vial (Corning, Acton, MA, USA) containing 500 ul 100 % glycerol and kept at -80 oC.
description
7 days growth
dev-stage
Stromatinia cepivora
http://purl.obolibrary.org/obo/NCBITaxon_38492
organism
M13 Forward
re_1
M13 Reverse
re_2
C2
strain
micelium
tissue-type
HR Forwar Sustractive Library 1
title
Pasmid
v_type
pCR2.1 TOPO TA
vector