LIBEST_020637
External Id
SAMN00152433
External Id
Colegio de Postgraduados, Orientacion en Fitopatologia
INSDC center name
2006-11-20T00:00:00Z
INSDC first public
2022-05-05T21:08:31.127Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS20623668
SRA accession
2 ul PCR fragments were ligated with 1 ul pCR4-TOPO cloning vector according to the manufacturer's instructions Invitrogen, Carlsbad, CA, USA). 4 ul ligation reaction solutions were transformed into 50 ul E. coli chemically competent cells strain TOP 10 F (Promega). The transformation culture was plated on Petri dishes containing LB/ampicillin, and colonies were screened for insert fragment. Selected colonies containing recombinant plasmid were inoculated separately into 5 ml LB/ampicillin/kanamycin solution and it was shaken at 37 oC overnight. Then 500 ul each culture were added into 2 ml cryogenic vial (Corning, Acton, MA, USA) containing 500 ul 100 % glycerol and kept at -80 oC.
description
24 hours germination in presence of water
dev-stage
Stromatinia cepivora
http://purl.obolibrary.org/obo/NCBITaxon_38492
organism
M13 Forward
re_1
M13 Reverse
re_2
Rancho El Colorado, Cortazar
strain
Sclerotia
tissue-type
YRP Non-Erumpent Sustractive Library
title
Plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pCR4 TOPO
vector