SAMN00167928
External Id
LIBEST_024926
External Id
DOE Joint Genome Institute
INSDC center name
2009-07-17T00:00:00Z
INSDC first public
2022-10-14T21:06:42.104Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS20623684
SRA accession
The library was made from oligo(dT) primed cDNA and cloned into the pCMV-SPORT6 vector (Invitrogen Corp., Carlsbad, CA). PolyA RNA was primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'- CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 2-8 kb using 1.1% agarose gel electrophoresis, then ligated into NotI and SalI-digested pCMV-SPORT6 vector. The library was constructed and sequencing performed at the DOE Joint Genome Institute (Walnut Creek, CA).
description
germinated spores were harvested after 4 hours growth on agar water
dev-stage
DH10B
lab-host
Melampsora laricis-populina
http://purl.obolibrary.org/obo/NCBITaxon_203908
organism
SalI
re_1
NotI
re_2
98AG31
strain
germinated and ungerminated urediniospores
tissue-type
CBWO Melampsora larici-populina germinated and ungerminated urediniospores (H)
title
pCMV-SPORT6
vector