LIBEST_020371
External Id
SAMN00166278
External Id
University of British Columbia
INSDC center name
2006-09-12T00:00:00Z
INSDC first public
2023-10-26T21:06:24.366Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS16610500
SRA accession
Mycelia for library construction were collected from solid media inoculated with a suspension containing 5x105 spores and were incubated for 7 days under ambient conditions. Mycelia were generated on media containing on 1% Difco MEA mixed with 1.5% (w/v) autoclaved agar amended with a blend of monoterpenes and diterpenes. mRNA was isolated from each tissue source independently and equal quantities of mRNA from each tissue were then pooled. cDNA libraries were normalized according to published methods [Bonaldo M.F. et al. (1996) Genome Research 6(9):791]. The MPB03 library was normalized to the Cot 5 level.
description
Harvested after 7 days growth
dev-stage
Leptographium clavigerum
http://purl.obolibrary.org/obo/NCBITaxon_226899
organism
EcoRI (5' end of cDNA)
re_1
XhoI (3' end of cDNA)
re_2
SLKw1407
strain
Mixed hyphal
tissue-type
MPB03
title
pBluescript II SK (+) XR
vector