SAMN00166848
External Id
LIBEST_023887
External Id
Nankai University
INSDC center name
2008-11-05T00:00:00Z
INSDC first public
2022-05-09T11:42:27.940Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS20623682
SRA accession
Cauliflower leaves were inoculated with Xcc at 4-5 leaves stage. Inoculated leaves were harvested 72h after inoculation. Total RNA and mRNA were extracted by Trizol(BBI) and PolyATract mRNA Isolation Systerm(Promega), respectively. SSH was performed using a PCR-Select cDNA Subtraction Kit (Clontech). The differentially expressed cDNA fragments were purified and cloned into pGEM-T Easy vectors(Promega) and transformed to E.coli JM109 competent cells, sequencing conducted by SP6 or T7 primer.
description
4-5 leaves, 3 days after inoculating by Xanthomonas campetris pv. campetris
dev-stage
Brassica oleracea var. botrytis
http://purl.obolibrary.org/obo/NCBITaxon_3715
organism
young leaves
tissue-type
SSH-cDNA Library of black rot infection in leaves of resistant variety compared with its susceptible near-isogenic line
title
botrytis
variety
pGEM-T easy
vector