2024-07-28
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114260541
External Id
EMBL
INSDC center name
2023-08-11T23:39:54Z
INSDC last update
public
INSDC status
ERS16248464
SRA accession
E-MTAB-13299:P15
Submitter Id
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Embryos were dissected at E12.5 post-coitum from the uteri of the females. Placentas were dissected in PBS + 0.1% BSA and the exterior decidua was peeled out. Clean placentas were bisected via transverse sectioning; one half was collected in a 1.5 ml tube containing 300 µl of 1x RNA Protection Reagent and snap-frozen. Homogenization of placentas and extraction of total RNA with Monarch Total RNA Miniprep Kit (NEB) followed by Turbo DNase treatment and final clean-up Library preparation was performed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, in combination with the NEBNext Poly(A) mRNA Magnetic Isolation Module for mRNA enrichment, according to the manufacturer’s instructions.
description
embryonic day 12.5
developmental stage
targeted mutation
genetic modification
Ogt[Y851A]/[WT]
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
placenta
organism part
Mus musculus
scientific_name
female
sex
FVB/Ncrl
strain
P15
title