LIBEST_027471
External Id
SAMN00716599
External Id
Plovdiv University
INSDC center name
2011-09-12T00:00:00Z
INSDC first public
2022-12-15T21:06:18.316Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS20720452
SRA accession
The isolation of total RNA is performed with mirVana miRNA isolation kit (Ambion), then a cDNA synthesis is performed according a standard protocol for the preparation of full-length enriched cDNA. A cDNA normalization is carried out, in order to amplify low copy transcripts, then only fragments greater than 0,5Kb are directionally ligated in the EcoRI and BamHI sites of the plasmid vector pBS II sk+ (amp. selection). Ligations are electroporated in T1 Phage resistant NEB 10-beta Electrocompetent E.coli cells (New England Biolabs).
description
mature plant
dev-stage
E.Coli
lab-host
Haberlea rhodopensis
http://purl.obolibrary.org/obo/NCBITaxon_121481
organism
EcoRI
re_1
BamHI
re_2
leaf
http://purl.obolibrary.org/obo/PO_0025034
tissue-type
Haberlea rhodopensis leaf cDNA library
title
plasmid
http://purl.obolibrary.org/obo/SO_0000155
v_type
pBS II
vector