SAMN00621739
External Id
Arkin group, Physical Biosciences Division, Lawrence Berkeley Lab
INSDC center name
2011-06-03T00:00:00Z
INSDC first public
2023-12-07T21:08:14.904Z
INSDC last update
SRS210922
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS210922
SRA accession
LS4D
media
Nitratidesulfovibrio vulgaris str. Hildenborough
http://purl.obolibrary.org/obo/NCBITaxon_882
organism
We used Terminator 5'-Phosphate-Dependent Exonuclease (Epicentre) to try to remove 5'-monophosphate (degraded) transcripts and then converted 5'-triphosphate ends to 5'-monophosphate ends with RNA 5' Polyphosphatase (Epicentre). Between each enzymatic reaction of this library, RNA was purified using Agencourt RNAClean XP beads. Molecules smaller than 100 nucleotides and unligated adaptors were mostly lost in these clean-up reactions.
protocol
DvH grown in defined lactate/sulfate media
source_name
Hildenborough
strain
Terminator 5'-Phosphate-Dependent Exonuclease and RNA 5' Polyphosphatase prep DvH_LS4D_5RNASeq_PPP
title
type strain of Desulfovibrio vulgaris
type material