2024-07-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112786120
External Id
Bioinformatician
INSDC center name
2023-03-17T11:24:35Z
INSDC last update
public
INSDC status
ERS14786905
SRA accession
E-MTAB-12761:Sample 8
Submitter Id
16
week
age
ArrayExpress
broker name
peritoneal macrophage
cell_type
house mouse
common name
Protocols: Peritoneal macrophages were obtained by intraperitoneal injection of cold PBS from Ldlr−/− mice reconstituted with Lyz2Cre+/− Nrp1flox/flox or Lyz2Cre+/− Nrp1+/+ bone marrow. Peritoneal exudates were allowed to adhere to the 6-well plates by culturing them for 2 hrs at 37°C. Animals were perfused with cold PBS to remove any trace of blood. Aortas were harvested and cleaned of contaminated tissues under a dissection microscope 16 wks mouse continuous high fat diet with Nrp1 WT vs KO Align to GRCm39 reference genome using star (version 2.6.1d) RNA extraction and residual DNA removal upon DNase digestion was performed with the RNeasy mini kit (Qiagen) according to the manufacturer’s instructions. After quality check with Agilent Bioanalyser 2100 system, total RNA was made into sequencing libraries. Takara’s SMART-Seq v4 Ultra Low Input RNA Kit.
description
adult
developmental stage
cwd continuous high fat diet
diet
normal
disease
Ldlr−/− mice reconstituted with Lyz2Cre+/− Nrp1flox/flox
genotype
KO_4
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
bone marrow
organism part
Nrp1 knockout
phenotype
Mus musculus
scientific_name
male
sex
LDLR-/-
strain
Sample 8
title