SAMN01818456
External Id
Ohio State University
INSDC center name
2013-11-01T07:44:08.647Z
INSDC first public
2023-10-17T16:06:02.830Z
INSDC last update
SRS376801
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS376801
SRA accession
Pupae of bumble bee used for sequencing were selected from USDA-ARS Bee Lab. The pupae were killed directly by immersion in RNAlater solution and submerged in approximately 5 volumes of RNAlater solution for RNA preservation. RNA was extracted from 20 pupae, 500 ng cRNAs were used to construct cDNA libraries. 454 pyrosequencing were completed by 454 Life Science/Roche platform on the GS-FLX platform. The sequences were then aligned and de novo assembled by software CLC Genomics Workbench to generate non-redundant contigs and singletons
description
Bombus huntii
http://purl.obolibrary.org/obo/NCBITaxon_85661
organism
Transcritptome Analsysis of Bomble Bee at Different Life Stages
title