SAMN01818457
External Id
Ohio State University
INSDC center name
2013-11-01T07:44:11.497Z
INSDC first public
2023-10-17T16:06:02.850Z
INSDC last update
SRS376802
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS376802
SRA accession
Adult males of bumble bee used for sequencing were selected from USDA-ARS Bee Lab. The bees were first killed by liquid nitrogen, then submerged in approximately 5 volumes of RNAlater solution for RNA preservation. RNA was extracted from 2 adult males. 500 ng mRNAs were used to construct cDNA libraries. 454 pyrosequencing were completed by 454 Life Science/Roche platform on the GS-FLX platform. The sequences were then aligned and de novo assembled by software CLC Genomics Workbench to generate non-redundant contigs and singletons
description
Bombus huntii
http://purl.obolibrary.org/obo/NCBITaxon_85661
organism
Transcritptome Analsysis of Bomble Bee at Different Life Stages
title