2024-07-31
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112797980
External Id
Bioinformatician
INSDC center name
2023-03-20T16:28:04Z
INSDC last update
public
INSDC status
ERS14805526
SRA accession
E-MTAB-12759:Sample 11
Submitter Id
16
week
age
ArrayExpress
broker name
macrophage
cell_type
house mouse
common name
Protocols: Aortas were harvested and cleaned of contaminated tissues. Aortas were subjected to enzymatic digestion method and single cell suspensions of aortic cells were prepared. Cell suspensions were incubated with Zombie Aqua™ Fixable Viability Kit (BioLegend) and then cells were stained with fluorescently labelled anti-mouse antibodies against CD45, CD11b and CD64. Aortic macrophages were identified as CD45+CD11b+CD64+. Animals were perfused with cold PBS to remove any trace of blood. Aortas were harvested and cleaned of contaminated tissues under a dissection microscope 16 wks mouse high fat diet. Align to GRCm39 reference genome using star (version 2.6.1d) RNA extraction and residual DNA removal upon DNase digestion was performed with the RNeasy mini kit (Qiagen) according to the manufacturer’s instructions. After quality check with Agilent Bioanalyser 2100 system, total RNA was made into sequencing libraries. Takara’s SMART-Seq v4 Ultra Low Input RNA Kit.
description
adult
developmental stage
cWD
diet
LDLR-/-
genotype
iWD_2_2
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
heart
organism part
Mus musculus
scientific_name
male
sex
LDLR-/-
strain
Sample 11
title