SAMN01818461
External Id
Ohio State University
INSDC center name
2013-11-01T07:44:10.240Z
INSDC first public
2023-10-17T16:06:02.930Z
INSDC last update
SRS376806
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS376806
SRA accession
Egg-laying queen of bumble bee used for sequencing were selected from USDA-ARS Bee Lab. The bee was first killed by liquid nitrogen, then submerged in approximately 5 volumes of RNAlater solution for RNA preservation. RNA was extracted from one egg-laying queen. 500 ng mRNAs were used to construct cDNA libraries. 454 pyrosequencing were completed by 454 Life Science/Roche platform on the GS-FLX platform. The sequences were then aligned and de novo assembled by software CLC Genomics Workbench to generate non-redundant contigs and singletons
description
Bombus huntii
http://purl.obolibrary.org/obo/NCBITaxon_85661
organism
Transcritptome Analsysis of Bomble Bee at Different Life Stages
title