2024-08-01
ENA first public
ERC000011
ENA-CHECKLIST
Centre of Marine Sciences (CCMAR)
INSDC center name
public
INSDC status
ERS20522346
SRA accession
E-MTAB-14218:Sample 3
Submitter Id
ArrayExpress
broker name
not collected
collection date
Senegalese sole
common name
Protocols: By the end of the experiment, 3 male fish per tank (2 tanks per group) were randomly sacrificed with a lethal dose of anesthesia (1000 ppm phenoxyethanol) and testis were immediately dissected and frozen in liquid nitrogen. Testis samples were stored at -80 ºC for RNA extraction and further RNA-Seq quantification. The gonads collected were homogenized in 1 mL of TRI Reagent® (Sigma-Aldrich, Switzerland) using a tissue-lyzer Star-Beater (VWR). Total RNA was extracted following the instructions of the manufacturer and the obtained pellet was resuspended in RNase-free distilled water (Sigma-Aldrich, Switzerland). Total RNA concentrations were measured with a Nanodrop One-C spectrophotometer (Thermo Fisher Scientific, Washington, USA) and adjusted to 1 µg/µL. Total RNA quality and integrity were checked using a 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA), and only samples with an RNA integrity number (RIN) > 8 were considered for sequencing. A total of 12 libraries were prepared using a TruSeq™ RNA Library Preparation Kit v2 of Illumina (Illumina, USA).
description
Breeder
developmental stage
control
diet
not collected
geographic location (country and/or sea)
3
individual
not applicable
isolate
Solea senegalensis
organism
Solea senegalensis
organism
testis
organism part
Solea senegalensis
scientific_name
Male
sex
Sample 3
title