2024-08-01
ENA first public
ERC000011
ENA-CHECKLIST
Jikei University School of Medicine
INSDC center name
public
INSDC status
ERS20444932
SRA accession
E-MTAB-14212:Jurkat_RELA1
Submitter Id
ArrayExpress
broker name
Jurkat
cell_line
not collected
collection date
human
common name
Protocols: Jurkat and MT-2 cells were provided by the Japan National Institute of Biomedical Innovation, Health and Nutrition, Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). The cell lines were cultured in Roswell Park Memorial Institute medium (Life Technologies) supplemented with 10% fetal bovine serum (Gibco). Mycoplasma testing (MycoAlert Quick Mycoplasma Detection Kit, Lonza) was routinely conducted. Mycoplasma contamination was not detected during the study period.  RNA was extracted from the 1 x106 cells by QIAzol Lysis Reagent (QIAGEN). Concentrations of nucleic acids were measured by nanodrop (all experiments) and/or Qubit HS RNA-kit (only for RNA-seq). RNA quantity and quality were measured by Agilent 2100 Bioanalyzer (Agilent technologies). RNA integration values (RIN) >8 were considered to proceed to the further analysis. RNA-seq libraries were generated using Illumina TruSeq Stranded Total RNA kits. The resultant cDNA libraries were quantified via Qubit and assessed for quality via Bioanalyzer.
description
RELA knockout
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
Jurkat_RELA1
title