2024-08-06
ENA first public
ERC000014
ENA-CHECKLIST
114
% of predicted
FEV1
IMU
INSDC center name
public
INSDC status
9.8
L
LCI
ERS20837353
SRA accession
MU43_V06_TS
Submitter Id
Host-associated
broad-scale environmental context
human-associated
broad-scale environmental context
zAMP
chimera check software
2018-06-13
collection date
No
cpneumoniae at visit
respiratory system
description
respiratory system
environmental medium
Throat swab
environmental medium
Switzerland
geographic location (country and/or sea)
46.52522
DD
geographic location (latitude)
6.64245
DD
geographic location (longitude)
5.4
years
host age
male
host sex
MU43
host subject id
amplicon sequencing
investigation type
paired-end
library construction method
PCR
library screening strategy
79
nM
library yield
Human
local environmental context
Throat
local environmental context
Cystic fibrosis
lung/pulmonary disorder
No
mpneumoniae at visit
16S Metagenomic Sequencing Library Preparation (Part. #15044223 Rev. B), Illumina (San Diego, United States)
nucleic acid amplification
MagNA Pure 96 Roche (Basel, Switzerland)
nucleic acid extraction
respiratory tract metagenome
organism
initial denaturation:95degC_5min; denaturation:95degC_0.5min; annealing:55degC_0.5min; elongation:72degC_0.5min; final elongation:72degC_5min; total cycles:25
pcr conditions
FWD:CCTACGGGNGGCWGCAG REV:GACTACHVGGGTATCTAATCC
pcr primers
MUCOVIB
project name
Unexpected associations between respiratory viruses and bacteria with Pulmonary Function Testing in children suffering from Cystic Fibrosis (MUCOVIB study)
project title
respiratory tract metagenome
scientific_name
Illumina MiSeq
sequencing method
16S rRNA
target gene
V3V4
target subfragment
MU43_V06_TS
title
Regular visit
visit status