2024-08-06
ENA first public
ERC000011
ENA-CHECKLIST
Department of Medicine 3 – Rheumatology and Immunology, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Uniklinikum Erlangen
INSDC center name
public
INSDC status
ERS20850419
SRA accession
E-MTAB-14339:Sample 3
Submitter Id
53
year
age
ArrayExpress
broker name
synovial cell
cell_type
not collected
collection date
human
common name
Protocols: Fresh frozen human synovial tissues were thawed, thoroughly minced and digested in RPMI 1640 containing 2 mg ∙ ml−1 collagenase D (Roche), 0.2 mg ∙ ml−1 Dispase II (Sigma-Aldrich) and 0.2 mg ∙ ml−1 DNase I (Roche). Samples were incubated three times at 37 °C for 20 min with constant shaking (2,000 rpm) on a thermal shaker (Eppendorf). After 20 min, the supernatant was collected, filtered (70 µm) and fresh digestion medium was added. The digestion was repeated for a total of 3 times. RBC lysis was performed using RBC lysis buffer (BioLegend) according to the manufacturer’s recommendations. The resulting cell suspensions were filtered (30 µm) and counted in trypan blue using a Neubauer counting chamber (Brand).  extracted with 10xGenomics Chromium Next GEM Single Cell 3ʹReagent Kits v3.1 (Dual Index) constructed with 10xGenomics Chromium Next GEM Single Cell 3ʹReagent Kits v3.1 (Dual Index) library construction kit
description
adult
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
synovial membrane
organism part
Homo sapiens
scientific_name
male
sex
Sample 3
title