2024-08-10
ENA first public
ERC000011
ENA-CHECKLIST
Johns Hopkins University School of Medicine
INSDC center name
public
INSDC status
ERS20619908
SRA accession
E-MTAB-14288:WT1
Submitter Id
6
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Tregs were isolated from mice and cultured in vitro for 3 days with activation using anti-CD3/CD28 beads.  Total RNA was extracted using the Trizol method. Following homogenization with Trizol, chloroform was added, followed by centrifugation. The aqueous phase was used for further isolation of RNA using isopropanol precipitation. Novogene Corporation Inc prepared the RNA-seq 250-300 bp insert cDNA library.
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
spleen
organism part
Mus musculus
scientific_name
male
sex
664
strain
WT1
title