2024-08-12
ENA first public
ERC000011
ENA-CHECKLIST
Wallenberg Center for Molecular Medicine (WCMM) Department of Biomedical and Clinical Sciences  Division of Molecular Medicine and Virology  Linköping University, Sweden
INSDC center name
public
INSDC status
ERS20850341
SRA accession
E-MTAB-14338:1_RO
Submitter Id
ArrayExpress
broker name
mESC
cell_line
mouse embryonic stem cell
cell_type
not collected
collection date
house mouse
common name
Protocols: 3 biological replicates of 250,000 cells/replicate were processed for each condition. Nuclei were pelleted and frozen after extraction in an isopropyl chamber, and then later thawed on ice to be processed for CUT&RUN in parallel.  CUT&RUN LoV-U for H3K4me was performed as described in (PMID: 36355069), with the following modifications. Antibodies used included anti-H3K4me (antibodies online, ABIN3023251) and anti-rabbit IgG (antibodies online, ABIN101961) at 1:100 dilutions. Library preparation was done using the KAPA Hyper Prep Kit for Illumina sequencing (Cat. #KK8504, KAPA Biosystems) following the manufacturer’s guidelines with modifications, as described in (PMID: 36355069).
description
embryo stage
developmental stage
none
disease
LSD1 KO
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
blastocyst
organism part
Mus musculus
scientific_name
WT LSD1 OE
stimulus
1_RO
title